For the production of industrial enzymes, recombinant antibody fragments and biopolymers in bacterial systems at high efficiency, and the selection of theranostic aptamers, we focus on genetic modifications, design of the production process in a bioreactor, mathematical modeling of metabolic reactions or efficient purification of the recombinant product.
• PCR device
• Nano-spectrophotometer
• Mini-Bioreactor System
• Incubator
• Shaking incubator
• Refrigerated centrifuge (rotor suitable for 2 ml, 15 ml and 50 ml tubes)
• Mini centrifuge (rotor suitable for 0.5 and 2 ml tubes)
• UV-Vis Spectrophotometer
• Laminar sterile cabinet
• Horizontal electrophoresis system
• Vertical electrophoresis and Western transfer module
• Gel image analysis system
• Gel imaging tray
• Microplate reader
• Ultrasonic homogenizer
• Vortex
• 3D shaker
• pH meter
• Ultra-pure water device
• Autoclave
• 4 ° C cooler
• -20 ° C freezer
• -86 ° C freezer
• Microwave oven
• UPS
• Assoc. Prof. Dr. Eda Çelik-Akdur
• Res. Assist. Dr. Murat Akdoğan (Chemical Eng.)
• Res. Assist. Dr. İlkay Koçer Kuloğlu (Chemical Eng.)
• Dilek Şahinbaş (Bioeng., PhD student)
• Emine Altun (Bioeng., PhD student, as co-advisor)
• Selçuk Uğurlu (Chemical Eng., MSc student)
• Selen Cilasun (Bioeng., MSc student)
• Nerve Cansu İşeri (Bioeng., MSc student)
• “Process Development for the Production of Anti-HER2 Antibody Fragment in Bacterial Soluble Form”, HÜ-BAP-FBA-2021-18857, Principal Investigator, 10/03/2021-12/09/2022.
• "Development of Aptamer Specific to Staphylococcus epidermidis, an Orthopedic Implant-borne Infection Factor, and Investigation of the Effect of Aptamer on Biofilm Formation in Vitro Conditions", TÜBİTAK Project No: 118Z798, Researcher, 01/03/2019, 01/03/2022.
• "Production and Genetic Modification of Recombinant anti-TNF and anti-IL6 Antibody Fragments for Diagnosis of Cytokine Storm", TÜBİTAK Project No:121M179, Advisor, 01/07/2021-01/07/2022.
• "Development of aptamer for medical diagnosis and specific for osteosarcoma with cell-SELEX method", HÜ-BAP- FHD-2018-17245, Principal Investigator, 08 / 2018-07 / 2019, Budget: 30 000 TL.
• “Biopolymer Production and Characterization with Recombinant Bacillus megaterium”, HÜ-BAP-FBB-2016-11851, Principal Investigator, 11 / 2016–06 / 2019, Budget: 145 000 TL.
• "Synthesis of Magnetic Silica Microspheres in Functional Group Form and Determination of Biotechnological Application Performance", TÜBİTAK Project No: 115M615, Advisor, 01/10/2015 - 01/04/2017.
• “Increasing the Composting Efficiency of Antimicrobial Polymers by Using Dominant Species”, TÜBİTAK Project No: 114Y559, Researcher, 01/05/2015 - 01/05/2017. Budget: 240 000 TL.
• “Production of New Generation Cellulase Enzymes for Biofuels Sector in Recombinant Escherichia coli Bacteria”, TÜBİTAK Project No: 114M133, Principal Investigator, 15/11/2014 - 15/02/2016. Budget: 60 000 TL.
• “Development of Glyco-Phage Arrays as a Novel High-Throughput Method for Glycomics”, European Union Project No: FP7-PEOPLE-2012-CIG-322096, Career Project Leader and Fellow, 01/10/2012 - 30/09 / 2016. Budget: 100 000 Euros.
A. Articles Published in Journals Scanned in SCI Index
. Tsogtbaatar K., Sousa D.A., Ferreira D., Tevlek A., Aydın H.M., Çelik E., Rodrigues L., 2022. In vitro selection of DNA aptamers against human osteosarcoma. Investigational New Drugs, 40(1): 172-181.
• Koçer İ., Cox E.C., DeLisa M.P., Çelik E., 2021. Effects of variable domain orientation on anti-HER2 single-chain variable fragment antibody expressed in the Escherichia coli cytoplasm. Biotechnology Progress, 37 (2), e3102.
• Akdoğan M., Çelik E., 2021. Enhanced production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors. Bioprocess and Biosystems Engineering, 44 (2): 403-416.
• Altun E., Çelik E., Yavuz Ersan H., 2020. Tailoring the Microbial Community for Improving the Biodegradation of Chitosan Films in Composting Environment, Journal of Polymers and the Environment, 28: 1548–1559.
• Yıldırım D, Kip Ç, Tsogtbaatar K, Koçer İ, Çelik E, Tuncel A, 2020. Microfluidic immobilized metal aff chromatography based on Ti (IV) -decorated silica microspheres for purification of phosphoproteins. Journal of Chromatography B, 1140: 122010.
• Gökçal B, Kip Ç, Şahinbaş D, Çelik E, Tuncel A, 2020. Silica microspheres functionalized with the iminodiacetic acid / copper (II) complex as a peroxidase mimic for use in metal affinity chromatography-based colorimetric determination of histidine-tagged proteins . Microchimica Acta, 187: 121.
• Kip Ç, Babacan-Tosun R, Alpaslan S, Koçer I, Çelik E, Tuncel A, 2019. Ni (II) -decorated porous titania microspheres as a stationary phase for column chromatography applications: Highly selective purification of hemoglobin from human blood. Talanta, 200, 100-106.
• Kip Ç, Gülüşür H, Çelik E, Usta DD, Tuncel A, 2019. Isolation of RNA and beta-NAD by phenylboronic acid functionalized, monodisperse-porous silica microspheres as sorbent in batch and microfluidic boronate affinity systems. Colloids and Surfaces B: Biointerfaces, 174, 333-342.
Akdoğan M, Çelik E, 2018. Purification and characterization of polyhydroxyalkanoate (PHA) from Bacillus megaterium strain using various dehydration techniques. Journal of Chemical Technology & Biotechnology. 93, 8, 2018, 2292-2298.
• Salimi K, Usta DD, Koçer I, Çelik E, Tuncel A, 2018. Protein A and protein A / G coupled magnetic SiO2 microspheres for affinity purification of immunoglobulin G. International Journal of Biological Macromolecules. 111, 2018, 178-185.
• Salimi K, Usta DD, Koçer I, Çelik E, Tuncel A, 2017. Highly flood ective magnetic affinity purification of histidine-tagged proteins by Ni2 + carrying monodisperse composite microspheres. RSC Advances, 7, 8718-8726.
• Yıldırım Z, Çelik E, 2017. Periplasmic and extracellular production of cellulase from recombinant Escherichia coli cells. Journal of Chemical Technology & Biotechnology, 92, 2, 319-324.
B. Papers presented in national and international scientific meetings and published in the proceeding book
• Koçer İ., Şahinbaş D, Cilasun S. Çelik E., 5-7 / 03/2020. Production of ScFv Antibody Fragments and Their Applications. International Biotechnology Congress (BIOTürkiye-2020), Istanbul, Turkey, Abstract Book, P39 (oral presentation).
• Çağdaş-Tunalı, B., Çelik E., Türk M., 17-20 / 10/2019. Development of hyaluronic acid-polyethylenimine delivery system for gene silencing in cancer treatment. 24th International Biomedical Science & Technology Symposium (BIOMED 2019), İzmir, Turkey, Abstract Book, p78.
• Altun, E., Çelik E., Yavuz Ersan H., 2-4 / 5/2018. Biodegradation Behavior of Two Different Chitosan Films under Controlled Composting Environment, Eurasia 2018 Waste Management Symposium, YTU Davutpaşa Congress Center, İstanbul, p804-808.
• Çağdaş-Tunalı, B., Çelik E., Türk M., 12-14 / 5/2017. Hyaluronic acid-polyethylenimine delivery system for gene silencing, 22nd International Biomedical Science & Technology Symposium (BIOMED 2017), Ankara, Turkey, Abstract Book p065. (poster)
• Çelik E. 5-7 / 10/2016. Glycophage based arrays as an alternative biosensor for pathogen detection. 3rd International Congress on Biosensors, Ankara, Turkey, Abstract book OP0210.
• Yıldırım Z., Çelik E. 23-26 / 8/2016. Production of recombinant cellulase in the glycoprotein structure in Escherichia coli bacteria. 12. National Chemical Engineering Congress (UKMK2016). Izmir Turkey. Abstract Book p15 (oral presentation).
• Altun E., Çelik E., Yavuz Ersan H. 23-26 / 8/2016. Investigation of the degradation mechanism of chitosan films in controlled compost reactors. 12. National Chemical Engineering Congress (UKMK2016). İzmir, Turkey, Abstract Book p258.
• Akdogan M., Çelik E. 26-29 / 9/2016. Production and characterization of poly-3-hydroxybutyrate (PHB) under different dehydration techniques by Bacillus megaterium strain. 15th International Symposium on Biopolymers (ISBP2016), Madrid, Spain. Abstract Book p58. (poster)
• Çelik E., 3-6 / 7/2016. Production of glycosylated recombinant proteins and phage particles in Escherichia coli. 17th European Congress on Biotechnology (ECB2016), Krakow, Poland. New Biotechnology 33, S44. (oral presentation)
• Usta D.D., Salimi K., Koçer I., Çelik E., Tuncel A., 19-24 / 6/2016. A New Magnetic-silica Based Sorbent for Isolation of Green Fluorescent Protein by Immobilized Metal Affinity Chromatography. 44th International Symposium & Exhibit on High Performance Liquid Phase Separations and Related Techniques (HPLC2016), San Francisco, USA. Abstract Book. (# P-Th-2432)
C. International books or chapters in books
Gündüz Ergün B., Celltogullari D., Öztürk S., Çelik E., Çalık P., 2019. Established and Upcoming Yeast Expression Systems. In: Gasser B., Mattanovich D. (eds), Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 1923, 1-74, Humana Press, New York, NY.
Official Web Page
yunus.hacettepe.edu.tr/~edacelik/res.htm