Micro and Nanomaterials Research Laboratory

About

Non-porous and porous micro / nanoparticles and metal-organic lattice structures are synthesized. Micro / nanomaterials are used in the synthesis of biomolecule isolation kits, artificial enzyme-based biosensors and combined photothermal / photodynamic / chemodynamic therapy agents within the scope of biotechnological and biomedical applications. In addition, the nanocatalyst / photocatalyst functions in clean energy generation, pollutant degradation and oxidation reactions are investigated.

Research Infrastructure

• Precision Balance
• Balloon Warmer
• Precision Balance
• Mechanical Mixer
• Oxygenmeter
• Optical Microscope
• Vacuum Pump
• Syringe Pump
• Vacuum Pump
• PH Meter
• Spectrophotometer
• Fluorescence Spectrophotometer
• Spectrophotometer
• BET Surface Area Analyzer
• Mechanical Mixer
• Mechanical Mixer
• Particle Measuring Device
• Vortex
• Rotator
• Vortex
• High Performance Liquid Chromatography (HPLC) System
• Ion Chromatography Device
• Capillary Electrochromatography Device
• Nano-Liquid Chromatography System
• Water bath
• Ultrasonic Bath
• Heated Magnetic Stirrer
• Heated Magnetic Stirrer
• Mechanical Mixer
• Heated Magnetic Stirrer
• Magnetic Stirrers
• Shaking Water Bath
• Balloon Warmer
• Evaporator
• Precision Balance
• Oven
• Multiple Magnetic Mixer
• Heated Magnetic Stirrer
• Thermalcycler PCR Device
• HPLC Pump Unit
• Syringe Pumps
• Vacuum Pump
• Ultra Pure Water Device
• Biosafety Cabinet
• Qubit Fluorometer

Researchers

• Prof. Dr. S. Ali TUNCEL
• Assoc. Dr. F. Çiğdem KİP
• Research Assist. Dr. K. Özlem HAMALOĞLU
• Lecturer Assist. M. Cihan DEMİR
• Res. See. Emotion YILDIRIM
• Res. See. Burcu Gokcal
• Rukiye BABACAN TOSUN (PhD Inst.)
• Eda ÖĞÜT (PhD Lecturer)
• Koray ÇAKIR (Graduate Inst.)

Ongoing Projects

• TÜBİTAK Concert Japan Project “Superior functionality: porous materials with composite / mixed matrix structure in membrane-based processes” Executive: Assoc. Enver Güler, Researchers: Prof. Dr. Ali Tuncel, Research Assist. Dr. Kadriye Özlem Hamaloğlu, Project Start: 01.04.2019, Budget: 426.325 TL

Completed Projects

• Hacettepe University Scientific Research Projects Coordination Unit Comprehensive Research Project “Development of peroxidase-microsime-based biosensors for protein determination” Project Code: FBA-2019-17337, Coordinator: Prof. Dr. Ali Tuncel, Researchers: Dr. Lecturer See. Çiğdem Kip, Inst. See. Mustafa Cihan Demir, Project Start: 05.02.2019, Budget: 104.583,92 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project, “Synthesis of Porous Oxidation Catalysts Containing Iridium or Ruthenium Active Center and Investigation of the Usability of Synthesized Catalysts in Water Oxidation” Project Code: FHD-2018-17235, Leader: Res. Assist. Dr. Kadriye Özlem Hamaloğlu, Researchers: Prof. Dr. Ali Tuncel, Dr. Lecturer Çiğdem Kip, Serap Ulu (M.Sc.), Rukiye Babacan Tosun (Ph.D.), Project Start and End: 26.06.2018 - 09.03.2020, Budget: 29.986,83 TL
• Hacettepe University Scientific Research Projects Coordination Unit Comprehensive Research Project, “Development of a new early stage tumor marker using molecular suppression and LC-MS / MS” Project Code: FBA-2017-12880, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Assoc. Dr. Ömür Çelikbıçak, Project Start and End: 07.03.2017 - 15.11.2019, Budget: 121.106.77 TL
• Hacettepe University Scientific Research Projects Coordination Unit International Research Cooperation Project "Development of functional group separation media and investigation of their chromatographic performances" Project Code: FBI-2017-13419, Coordinator: Dr. Lecturer Çiğdem Kip, Project Start and End: 30.01.2017 - 16.04.2018 Budget: 19.500 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project, “Development of hydrophilic ligand-bonded equi-size porous poly (TMSPM-co-EDMA) particle filled microcolons for hydrophilic interaction chromatography with thiolene reaction” Project Code: FHD-2016-8480, Coordinator: Dr . Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 18.10.2016 - 23.02.2018 Budget: 22.034,62 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project “Investigation of nucleoside isolation performance of Ti + 4 derivatized polydopamine coated titanium dioxide microspheres” Project Code: FHD-2016-13123, Coordinator: Res.Assist.Dr. Kadriye Özlem Hamaloğlu, Researchers: Prof. Dr. Ali Tuncel, Ebru Sağ (Ph.D.) Project Start and End: 26.12.2016 - 18.12.2017, Budget: 22.105,40 TL
• Hacettepe University Scientific Research Projects Coordination Unit Scientific Achievement Support “Determination of structural properties of hyaluronan HA nanoparticles containing covalently bound chondroitin sulfate (CS) and glycosaminoglycan (GAG) by developing CS-GAG-nHA and investigation of their effects on in vitro osteoarthritis model” Supervisor: Prof . Dr. Feza Korkusuz, Researchers: Inst. See. Elif Bilgiç, Şahin Ş (Graduate Inst.), Prof Dr. Ali Tuncel, Prof. Dr. Petek Korkusuz, Project Start and End: 21.12.2016 - 05.05.2017, Budget: 7219,80 TL
• TÜBITAK 1001 Project "Synthesis of organosilicon capillary monoliths with thiol-en chemistry and their use as stationary phase in nano-liquid chromatography" Project Code: 115Z250, Coordinator: Prof. Dr. Ali Tuncel, Advisor: Dr. Lecturer See. Çiğdem Kip, Researchers: Dr. Lecturer See. Kouroush Salimi, Inst. See. Mustafa Cihan Demir, Fatma Çambay (Ph.D.) Project Start and End: 01/11 / 2015-15 / 02/2018, Budget: 389.317 TL
• TÜBİTAK 1005 Project "Synthesis of Magnetic Silica Microspheres in Functional Group Form and Determination of Biotechnological Application Performance" Project Code: 115M615 Leader: Prof.Dr. Ali Tuncel, Advisor: Assoc. Dr. Eda CELIK AKDUR, Dr. Lecturer See. Çiğdem Kip Researchers: Dr. Lecturer See. Kouroush Salimi, Duygu Deniz USTA, Project Start and End: October 2015 - April 2017, Budget: 301.815 TL
• Hacettepe University Scientific Research Projects Coordination Unit Scientific Achievement Support “Synthesis of polymethacrylate-based monoliths carrying lanthanide ions functionalized with phosphoric acid for immobilized metal affinity micro chromatography” Project Code: FBB-2016-10476, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 27.04.2016 - 30.09.2016 Budget: 6000 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project "Development of magnetic photocatalysts for photocatalytic decomposition of 4-nitrophenol from organic pollutants" Project Code: FHD-2015-6266, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 08.05.2015 - 16.05.2016 Budget: 20,679.85 TL

Publications

• Hamaloglu K.O., Tosun R.B., Kayi H., Kavaklı P.A., Kavaklı C., Tuncel A. (2021). Monodisperse-porous cerium oxide microspheres carrying iridium oxide nanoparticles as a heterogeneous catalyst for water oxidation, Applied Surface Science, 547, Art. No: 149219.

• Tosun R.B., Hamaloglu K.O., Kavaklı C., Kavaklı P.A., Tuncel, A. (2021). Reusable water oxidation catalyst with dual active center for enhanced water oxidation: Iridium oxide nanoparticles immobilized on monodisperse-porous Mn5O8 microspheres, International Journal of Hydrogen Energy, 46, 15482-15496.

• Hamaloglu K.O., Tosun R.B., Ulu S., Kavaklı C., Kavaklı P.A., Kip C., Tuncel, A. (2021). Monodisperse-porous cerium oxide microspheres as a new support with appreciable catalytic activity for a composite catalyst in benzyl alcohol oxidation, New Journal of Chemistry, 45 (4) 2019-2029.

Kip C., Hamaloglu K.O., Demir C., Tuncel A. (2021) Recent trends in sorbents for bioaffinity chromatography, Journal of Separation Science, 44 (6) 1273-1291.

• Gökçal B., Hamaloğlu K. Ö., Kip Ç., Güngör S. Y., Büber E., Tuncel A. (2020). Glutathione detection in human serum using gold nanoparticle decorated, monodisperse porous silica microspheres in the magnetic form. Analytical Methods, 12 (43), 5219-5228.

• Tosun R. B., Hamaloğlu K. Ö., Kavaklı P. A., Kavaklı C., Tuncel A. (2020). A synergistic catalyst based on a multivalence monodisperse-porous microspheres with oxygen vacancies for benzyl alcohol oxidation. Molecular Catalysis, 497, Art. No: 111227.

• Yıldırım D., Gökçal B., Büber E., Kip Ç., Demir M. C., Tuncel, A. (2020). A new nanozyme with peroxidase-like activity for simultaneous phosphoprotein isolation and detection based on metal oxide affinity chromatography: Monodisperse-porous cerium oxide microspheres. Chemical Engineering Journal, 403, Art. No: 126357.

• Gökçal B., Kip Ç., Şahinbaş D., Çelik E., Tuncel A. (2020). Silica microspheres functionalized with the iminodiacetic acid / copper (II) complex as a peroxidase mimic for use in metal affinity chromatography-based colorimetric determination of histidine-tagged proteins. Microchimica Acta, 187 (2), 1-9.

• Gökçal B., Kip Ç., Tuncel A. (2020). One-pot, direct glucose detection in human whole blood without using a dilution factor by a magnetic nanozyme with dual enzymatic activity. Journal of Alloys and Compounds, 843, Art. No: 156012.

• Kip Ç., Akbay E., Gökçal B., Savaş B. O., Onur M. A., Tuncel, A. (2020). Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 598, Art. No. 124812.

• Yıldırım, D., Kip, Ç., Tsogtbaatar, K., Koçer, İ., Çelik, E., Tuncel, A. (2020). Microfluidic immobilized metal affinity chromatography based on Ti (IV) -decorated silica microspheres for purification of phosphoproteins. Journal of Chromatography B, 1140, Art. No: 122010.

• Öğüt E., Kip Ç., Gökçal B., Tuncel A. (2019). Aggregation-resistant nanozyme containing accessible magnetite nanoparticles immobilized in monodisperse-porous silica microspheres for colorimetric assay of human genomic DNA. Journal of Colloid and Interface Science, 550, 90-98.

• Kip Ç., Tosun R. B., Alpaslan S., Koçer İ., Çelik E., Tuncel A. (2019). Ni (II) -decorated porous titania microspheres as a stationary phase for column chromatography applications: Highly selective purification of hemoglobin from human blood. Talanta, 200, 100-106.

• Tosun R. B., Kip Ç., Tuncel A. (2019). Polymeric template assisted synthesis of monodisperse-porous manganese oxide microspheres: a new nanozyme with oxidase-like activity allowing biomolecule determination via bimodal sensing. New Journal of Chemistry, 43 (47), 18505-18516.

• Süngü Ç., Kip Ç., Tuncel A. (2019). Molecularly imprinted polymeric shell coated monodisperse ‐ porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography. Journal of Separation Science, 42 (11), 1962-1971.

• Kip Ç., Liu S., Fu X., Tuncel A., Lämmerhofer, M. (2019). In-situ photopolymerized C4-functionalized organosilicon monoliths for reversed-phase protein separation in nano-liquid chromatography. Talanta, 198,333-336.

• Kip Ç., Gülüşür H., Çelik E., Usta D. D., Tuncel A. (2019). Isolation of RNA and beta-NAD by phenylboronic acid functionalized, monodisperse-porous silica microspheres as sorbent in batch and microfluidic boronate affinity systems. Colloids and Surfaces B: Biointerfaces, 174, 333-342.

• Hamaloğlu K. Ö., Sağ E., Kip Ç., Şenlik E., Kaya B. S., Tuncel, A. (2019). Magnetic-porous microspheres with synergistic catalytic activity of small-sized gold nanoparticles and titania matrix. Frontiers of Chemical Science and Engineering, 13 (3), 574-585.

• Salimi K., Kip Ç., Çelikbıçak Ö., Usta D. D., Pınar A., ​​Salih B., Tuncel A. (2019). Ti (IV) attached ‐ phosphonic acid functionalized capillary monolith as a stationary phase for in syringe ‐ type fast and robust enrichment of phosphopeptides. Biomedical Chromatography, 33 (6), Art. No: e4488.

• Salimi, K., Usta, D. D., Koçer, İ., Çelik, E., Tuncel, A. (2018). Protein A and protein A / G coupled magnetic SiO2 microspheres for affinity purification of immunoglobulin G. International Journal of Biological Macromolecules, 111, 178-185.

• Günal G., Kip Ç., Öğüt E. S., İlhan H., Kibar G., Tuncel A. (2018). Comparative DNA isolation behaviors of silica and polymer based sorbents in batch fashion: Monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation. Artificial Cells, Nanomedicine, and Biotechnology, 46 (1), 178-184.

• Demir C., Kip Ç., Tuncel A. (2018). Alkanethiol ‐ functionalized organosilicon monoliths for nano ‐ reversed ‐ phase liquid chromatography. Electrophoresis, 39 (22), 2919-2928.

• Hamaloğlu K. Ö., Sağ E., Bilir A., ​​Tuncel A. (2018). Monodisperse-porous titania microspheres and their gold decorated forms as new photocatalysts for dye degradation in batch fashion. Materials Chemistry and Physics, 207, 359-366.

• Salimi K., Usta D. D., Koçer İ., Çelik E., Tuncel A. (2017). Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2 + carrying monodisperse composite microspheres. RSC Advances, 7 (14), 8718-8726.

• Salimi K., Usta D. D., Çelikbıçak Ö., Pinar A., ​​Salih B., Tuncel A. (2017). Ti (IV) carrying polydopamine-coated, monodisperse-porous SiO2 microspheres with stable magnetic properties for highly selective enrichment of phosphopeptides. Colloids and Surfaces B: Biointerfaces, 153, 280-290.

• Kip Ç., Demir C., Tuncel A. (2017). One pot synthesis of carboxyl functionalized-polyhedral oligomeric siloxane based monolith via photoinitiated thiol-methacrylate polymerization for nano-hydrophilic interaction chromatography. Journal of Chromatography A, 1502, 14-23.

• Günal G., Kip Ç., Öğüt S. E., Usta D. D., Şenlik E., Kibar G., Tuncel A. (2017). Human genomic DNA isolation from whole blood using a simple microfluidic system with silica-and polymer-based stationary phases. Materials Science and Engineering: C, 74, 10-20.

• Hamaloğlu K. O., Sağ E., Tuncel A. (2017). Bare, gold and silver nanoparticle decorated, monodisperse-porous titania microbeads for photocatalytic dye degradation in a newly constructed microfluidic, photocatalytic packed-bed reactor. Journal of Photochemistry and Photobiology A: Chemistry, 332, 60-65.

• Salimi K., Usta D. D., Çelikbıçak Ö., Pınar A., ​​Salih B., Tuncel A. (2017). Highly selective enrichment of phosphopeptides by titanium (IV) attached monodisperse-porous poly (vinylphosphonic acid-co-ethylene dimethacrylate) microspheres. Journal of Chromatography A, 1496, 9-19.

• Kip Ç., Tuncel A. (2017). HILIC stationary phase based on monodisperse-porous polymethacrylate beads functionalized with zwitterionic molecular brushes. Chromatographia, 80 (4), 565-575.


B. International Congresses
• Gökcal B., Kip C., Tuncel A. Colorimetric method for ovalbumin detection using a new magnetic nanozyme. International Eurasian Conference on Biotechnology and Biochemistry, 2020.

• Yıldırım D., Kip C., Tuncel A. A novel nanozyme for colorimetric detection of L-histidine. VI. International Conference on New Trends in Chemistry, 2020.
• Tosun R., Hamaloğlu K. Ö., Tuncel A. Iridium oxide immobilized on cerium dioxide for enhanced water oxidation. VI. International Conference on New Trends in Chemistry, 2020.

• Ogut E., Kip C., Gökcal., Yıldırım D., Tuncel A. A new group of nanozymes for detection of some common biomolecules. HPLC 2019 - 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2019, Milan, Italy.

• Gökçal B., Kip Ç., Tosun R., Demir C., Tuncel A. Effects of process conditions on peroxidase-like activity of monodisperse-porous metal oxide microspheres modified with cupric oxide. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Gökçal B., Demir C., Tuncel A. Peroxidase-like activity of magnetic, monodisperse-porous SiO2 microspheres for sensing of proteins. 32. International Symposium on Chromatography 2018, Cannes, France.

• Kip Ç., Gökçal B., Demir C., Tuncel A. Effects of vitamins on the peroxidase like activity of monodisperse-porous MnO2 microspheres 5 μm in size. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Demir C., Tuncel A. Development of a new early stage tumor marker using molecular imprinting 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip F. Ç., Koçer İ., Çambay F., Çelik E., Tuncel A. A microfluidic system based on an organosilicon monolith for affinity separation of histidine-tagged proteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Süngü Ç., Kip Ç., Tuncel A. Boronate affinity separation of β-NAD via molecularly imprinted silica particles in batch and microfluidic systems. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Yıldırım D., Süngü Ç.Z., Tuncel A. Microfluidic system for immobilized metal affinity separation of phosphoproteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Alan B., Kip Ç., Tuncel A. Molecularly imprinted monodisperse-porous, silica microbeads for folic acid determination. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Alpaslan S., Tosun Babacan R., Kip C., Alan B., Tuncel A. Titania based immobilized metal affinity sorbent for the purification of histidine-rich proteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Demir M.C., Tuncel A. Hybrid stationary phases with retention independent separation performance in nano-hydrophilic interaction chromatography. HPLC 2017-45. International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Demir M.C., Nedjip G., Tuncel A. Preparation of phenylboronic acid functionalized-polyhedral oligomeric siloxane monolithic column via one-pot approach for nano liquid chromatography of small molecules. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Seperations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Demir M.C., Kuban A., Tuncel A. Alkanethiol functionalized organosilicon monoliths via one-pot thiol-methacrylate polymerization for nano-reversed phase chromatography. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Usta DD, Kip Ç., Salimi K., Celikbıçak Ö., Pınar A., ​​Salih B., Tuncel A. Phosphopeptide enrichment from human-serum via immobilized metal affinity chromatography using phosphonic acid functionalized capillary monolith as a stationary phase in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Durmaz M, Salimi K., Kuban A., Tuncel A. Phosphopeptide enrichment from human-serum via immobilized metal affinity chromatography using phosphonic acid functionalized capillary monolith as a stationary phase in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Nedjip G., Demir M.C., Tuncel A. Preparation of phenylboronic acid functionalized hybrid monolithic column for boronate affinity chromatography of diol compounds in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Salimi K., Usta D.D., Çelikbıçak Ö., Salih B., Tuncel A., Phosphopeptide enrichment using Ti (IV) attached monodisperse-porous poly (phosphonic acid) based microspheres. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Usta D.D., Salimi K., Kip Ç., Çelikkaya B., Tuncel A. Polydopamine coated, magnetic monodisperse-porous silica microspheres as a stationary phase for protein separation by immobilized metal affinity chromatography (IMAC). 44. International Symposium on High Performance Liquid Separations and Related Techniques, June 19-24th, 2016, San Fransisco, U.S.A.

• Kip Ç., Usta D.D., Salimi K., Gülüşür H., Tuncel A. Batch and microfluidic isolation of diol carrying biomolecules by a boronate affinity sorbent based on monodisperse-porous microspheres. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Usta D.D., Salimi K., Koçer İ., Çelik E., Tuncel A. A new magnetic-silica based sorbent for iolation of green fluorescent protein by immobilized metal affinity chromatography. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Kip Ç., Usta D.D., İpek Ö., Demir M.C., Tuncel A. Immobilized metal affinity microchromatography using phosphonic acid functionalized polymethacrylate based monolithic stationary phases carrying lantanide ions. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Demir M.C., Kip Ç., Tuncel A. Nano-HILIC applications of polyhedral oligomeric silsesquioxane based capillary monoliths functionalized with anionic and cationic ligands. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Sağ E., Hamaloğlu K.Ö., Demir M.C., Tuncel A. Ti (IV) functionalized monodisperse titania microspheres for nucleoside isolation by immobilized metal affinity chromatography. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

Links

About

Non-porous and porous micro / nanoparticles and metal-organic lattice structures are synthesized. Micro / nanomaterials are used in the synthesis of biomolecule isolation kits, artificial enzyme-based biosensors and combined photothermal / photodynamic / chemodynamic therapy agents within the scope of biotechnological and biomedical applications. In addition, the nanocatalyst / photocatalyst functions in clean energy generation, pollutant degradation and oxidation reactions are investigated.

Research Infrastructure

• Precision Balance
• Balloon Warmer
• Precision Balance
• Mechanical Mixer
• Oxygenmeter
• Optical Microscope
• Vacuum Pump
• Syringe Pump
• Vacuum Pump
• PH Meter
• Spectrophotometer
• Fluorescence Spectrophotometer
• Spectrophotometer
• BET Surface Area Analyzer
• Mechanical Mixer
• Mechanical Mixer
• Particle Measuring Device
• Vortex
• Rotator
• Vortex
• High Performance Liquid Chromatography (HPLC) System
• Ion Chromatography Device
• Capillary Electrochromatography Device
• Nano-Liquid Chromatography System
• Water bath
• Ultrasonic Bath
• Heated Magnetic Stirrer
• Heated Magnetic Stirrer
• Mechanical Mixer
• Heated Magnetic Stirrer
• Magnetic Stirrers
• Shaking Water Bath
• Balloon Warmer
• Evaporator
• Precision Balance
• Oven
• Multiple Magnetic Mixer
• Heated Magnetic Stirrer
• Thermalcycler PCR Device
• HPLC Pump Unit
• Syringe Pumps
• Vacuum Pump
• Ultra Pure Water Device
• Biosafety Cabinet
• Qubit Fluorometer

Researchers

• Prof. Dr. S. Ali TUNCEL
• Assoc. Dr. F. Çiğdem KİP
• Research Assist. Dr. K. Özlem HAMALOĞLU
• Lecturer Assist. M. Cihan DEMİR
• Res. See. Emotion YILDIRIM
• Res. See. Burcu Gokcal
• Rukiye BABACAN TOSUN (PhD Inst.)
• Eda ÖĞÜT (PhD Lecturer)
• Koray ÇAKIR (Graduate Inst.)

Ongoing Projects

• TÜBİTAK Concert Japan Project “Superior functionality: porous materials with composite / mixed matrix structure in membrane-based processes” Executive: Assoc. Enver Güler, Researchers: Prof. Dr. Ali Tuncel, Research Assist. Dr. Kadriye Özlem Hamaloğlu, Project Start: 01.04.2019, Budget: 426.325 TL

Completed Projects

• Hacettepe University Scientific Research Projects Coordination Unit Comprehensive Research Project “Development of peroxidase-microsime-based biosensors for protein determination” Project Code: FBA-2019-17337, Coordinator: Prof. Dr. Ali Tuncel, Researchers: Dr. Lecturer See. Çiğdem Kip, Inst. See. Mustafa Cihan Demir, Project Start: 05.02.2019, Budget: 104.583,92 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project, “Synthesis of Porous Oxidation Catalysts Containing Iridium or Ruthenium Active Center and Investigation of the Usability of Synthesized Catalysts in Water Oxidation” Project Code: FHD-2018-17235, Leader: Res. Assist. Dr. Kadriye Özlem Hamaloğlu, Researchers: Prof. Dr. Ali Tuncel, Dr. Lecturer Çiğdem Kip, Serap Ulu (M.Sc.), Rukiye Babacan Tosun (Ph.D.), Project Start and End: 26.06.2018 - 09.03.2020, Budget: 29.986,83 TL
• Hacettepe University Scientific Research Projects Coordination Unit Comprehensive Research Project, “Development of a new early stage tumor marker using molecular suppression and LC-MS / MS” Project Code: FBA-2017-12880, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Assoc. Dr. Ömür Çelikbıçak, Project Start and End: 07.03.2017 - 15.11.2019, Budget: 121.106.77 TL
• Hacettepe University Scientific Research Projects Coordination Unit International Research Cooperation Project "Development of functional group separation media and investigation of their chromatographic performances" Project Code: FBI-2017-13419, Coordinator: Dr. Lecturer Çiğdem Kip, Project Start and End: 30.01.2017 - 16.04.2018 Budget: 19.500 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project, “Development of hydrophilic ligand-bonded equi-size porous poly (TMSPM-co-EDMA) particle filled microcolons for hydrophilic interaction chromatography with thiolene reaction” Project Code: FHD-2016-8480, Coordinator: Dr . Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 18.10.2016 - 23.02.2018 Budget: 22.034,62 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project “Investigation of nucleoside isolation performance of Ti + 4 derivatized polydopamine coated titanium dioxide microspheres” Project Code: FHD-2016-13123, Coordinator: Res.Assist.Dr. Kadriye Özlem Hamaloğlu, Researchers: Prof. Dr. Ali Tuncel, Ebru Sağ (Ph.D.) Project Start and End: 26.12.2016 - 18.12.2017, Budget: 22.105,40 TL
• Hacettepe University Scientific Research Projects Coordination Unit Scientific Achievement Support “Determination of structural properties of hyaluronan HA nanoparticles containing covalently bound chondroitin sulfate (CS) and glycosaminoglycan (GAG) by developing CS-GAG-nHA and investigation of their effects on in vitro osteoarthritis model” Supervisor: Prof . Dr. Feza Korkusuz, Researchers: Inst. See. Elif Bilgiç, Şahin Ş (Graduate Inst.), Prof Dr. Ali Tuncel, Prof. Dr. Petek Korkusuz, Project Start and End: 21.12.2016 - 05.05.2017, Budget: 7219,80 TL
• TÜBITAK 1001 Project "Synthesis of organosilicon capillary monoliths with thiol-en chemistry and their use as stationary phase in nano-liquid chromatography" Project Code: 115Z250, Coordinator: Prof. Dr. Ali Tuncel, Advisor: Dr. Lecturer See. Çiğdem Kip, Researchers: Dr. Lecturer See. Kouroush Salimi, Inst. See. Mustafa Cihan Demir, Fatma Çambay (Ph.D.) Project Start and End: 01/11 / 2015-15 / 02/2018, Budget: 389.317 TL
• TÜBİTAK 1005 Project "Synthesis of Magnetic Silica Microspheres in Functional Group Form and Determination of Biotechnological Application Performance" Project Code: 115M615 Leader: Prof.Dr. Ali Tuncel, Advisor: Assoc. Dr. Eda CELIK AKDUR, Dr. Lecturer See. Çiğdem Kip Researchers: Dr. Lecturer See. Kouroush Salimi, Duygu Deniz USTA, Project Start and End: October 2015 - April 2017, Budget: 301.815 TL
• Hacettepe University Scientific Research Projects Coordination Unit Scientific Achievement Support “Synthesis of polymethacrylate-based monoliths carrying lanthanide ions functionalized with phosphoric acid for immobilized metal affinity micro chromatography” Project Code: FBB-2016-10476, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 27.04.2016 - 30.09.2016 Budget: 6000 TL
• Hacettepe University Scientific Research Projects Coordination Unit Rapid Support Project "Development of magnetic photocatalysts for photocatalytic decomposition of 4-nitrophenol from organic pollutants" Project Code: FHD-2015-6266, Coordinator: Dr. Lecturer Çiğdem Kip, Researchers: Prof. Dr. Ali Tuncel, Project Start and End: 08.05.2015 - 16.05.2016 Budget: 20,679.85 TL

Publications

• Hamaloglu K.O., Tosun R.B., Kayi H., Kavaklı P.A., Kavaklı C., Tuncel A. (2021). Monodisperse-porous cerium oxide microspheres carrying iridium oxide nanoparticles as a heterogeneous catalyst for water oxidation, Applied Surface Science, 547, Art. No: 149219.

• Tosun R.B., Hamaloglu K.O., Kavaklı C., Kavaklı P.A., Tuncel, A. (2021). Reusable water oxidation catalyst with dual active center for enhanced water oxidation: Iridium oxide nanoparticles immobilized on monodisperse-porous Mn5O8 microspheres, International Journal of Hydrogen Energy, 46, 15482-15496.

• Hamaloglu K.O., Tosun R.B., Ulu S., Kavaklı C., Kavaklı P.A., Kip C., Tuncel, A. (2021). Monodisperse-porous cerium oxide microspheres as a new support with appreciable catalytic activity for a composite catalyst in benzyl alcohol oxidation, New Journal of Chemistry, 45 (4) 2019-2029.

Kip C., Hamaloglu K.O., Demir C., Tuncel A. (2021) Recent trends in sorbents for bioaffinity chromatography, Journal of Separation Science, 44 (6) 1273-1291.

• Gökçal B., Hamaloğlu K. Ö., Kip Ç., Güngör S. Y., Büber E., Tuncel A. (2020). Glutathione detection in human serum using gold nanoparticle decorated, monodisperse porous silica microspheres in the magnetic form. Analytical Methods, 12 (43), 5219-5228.

• Tosun R. B., Hamaloğlu K. Ö., Kavaklı P. A., Kavaklı C., Tuncel A. (2020). A synergistic catalyst based on a multivalence monodisperse-porous microspheres with oxygen vacancies for benzyl alcohol oxidation. Molecular Catalysis, 497, Art. No: 111227.

• Yıldırım D., Gökçal B., Büber E., Kip Ç., Demir M. C., Tuncel, A. (2020). A new nanozyme with peroxidase-like activity for simultaneous phosphoprotein isolation and detection based on metal oxide affinity chromatography: Monodisperse-porous cerium oxide microspheres. Chemical Engineering Journal, 403, Art. No: 126357.

• Gökçal B., Kip Ç., Şahinbaş D., Çelik E., Tuncel A. (2020). Silica microspheres functionalized with the iminodiacetic acid / copper (II) complex as a peroxidase mimic for use in metal affinity chromatography-based colorimetric determination of histidine-tagged proteins. Microchimica Acta, 187 (2), 1-9.

• Gökçal B., Kip Ç., Tuncel A. (2020). One-pot, direct glucose detection in human whole blood without using a dilution factor by a magnetic nanozyme with dual enzymatic activity. Journal of Alloys and Compounds, 843, Art. No: 156012.

• Kip Ç., Akbay E., Gökçal B., Savaş B. O., Onur M. A., Tuncel, A. (2020). Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 598, Art. No. 124812.

• Yıldırım, D., Kip, Ç., Tsogtbaatar, K., Koçer, İ., Çelik, E., Tuncel, A. (2020). Microfluidic immobilized metal affinity chromatography based on Ti (IV) -decorated silica microspheres for purification of phosphoproteins. Journal of Chromatography B, 1140, Art. No: 122010.

• Öğüt E., Kip Ç., Gökçal B., Tuncel A. (2019). Aggregation-resistant nanozyme containing accessible magnetite nanoparticles immobilized in monodisperse-porous silica microspheres for colorimetric assay of human genomic DNA. Journal of Colloid and Interface Science, 550, 90-98.

• Kip Ç., Tosun R. B., Alpaslan S., Koçer İ., Çelik E., Tuncel A. (2019). Ni (II) -decorated porous titania microspheres as a stationary phase for column chromatography applications: Highly selective purification of hemoglobin from human blood. Talanta, 200, 100-106.

• Tosun R. B., Kip Ç., Tuncel A. (2019). Polymeric template assisted synthesis of monodisperse-porous manganese oxide microspheres: a new nanozyme with oxidase-like activity allowing biomolecule determination via bimodal sensing. New Journal of Chemistry, 43 (47), 18505-18516.

• Süngü Ç., Kip Ç., Tuncel A. (2019). Molecularly imprinted polymeric shell coated monodisperse ‐ porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography. Journal of Separation Science, 42 (11), 1962-1971.

• Kip Ç., Liu S., Fu X., Tuncel A., Lämmerhofer, M. (2019). In-situ photopolymerized C4-functionalized organosilicon monoliths for reversed-phase protein separation in nano-liquid chromatography. Talanta, 198,333-336.

• Kip Ç., Gülüşür H., Çelik E., Usta D. D., Tuncel A. (2019). Isolation of RNA and beta-NAD by phenylboronic acid functionalized, monodisperse-porous silica microspheres as sorbent in batch and microfluidic boronate affinity systems. Colloids and Surfaces B: Biointerfaces, 174, 333-342.

• Hamaloğlu K. Ö., Sağ E., Kip Ç., Şenlik E., Kaya B. S., Tuncel, A. (2019). Magnetic-porous microspheres with synergistic catalytic activity of small-sized gold nanoparticles and titania matrix. Frontiers of Chemical Science and Engineering, 13 (3), 574-585.

• Salimi K., Kip Ç., Çelikbıçak Ö., Usta D. D., Pınar A., ​​Salih B., Tuncel A. (2019). Ti (IV) attached ‐ phosphonic acid functionalized capillary monolith as a stationary phase for in syringe ‐ type fast and robust enrichment of phosphopeptides. Biomedical Chromatography, 33 (6), Art. No: e4488.

• Salimi, K., Usta, D. D., Koçer, İ., Çelik, E., Tuncel, A. (2018). Protein A and protein A / G coupled magnetic SiO2 microspheres for affinity purification of immunoglobulin G. International Journal of Biological Macromolecules, 111, 178-185.

• Günal G., Kip Ç., Öğüt E. S., İlhan H., Kibar G., Tuncel A. (2018). Comparative DNA isolation behaviors of silica and polymer based sorbents in batch fashion: Monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation. Artificial Cells, Nanomedicine, and Biotechnology, 46 (1), 178-184.

• Demir C., Kip Ç., Tuncel A. (2018). Alkanethiol ‐ functionalized organosilicon monoliths for nano ‐ reversed ‐ phase liquid chromatography. Electrophoresis, 39 (22), 2919-2928.

• Hamaloğlu K. Ö., Sağ E., Bilir A., ​​Tuncel A. (2018). Monodisperse-porous titania microspheres and their gold decorated forms as new photocatalysts for dye degradation in batch fashion. Materials Chemistry and Physics, 207, 359-366.

• Salimi K., Usta D. D., Koçer İ., Çelik E., Tuncel A. (2017). Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2 + carrying monodisperse composite microspheres. RSC Advances, 7 (14), 8718-8726.

• Salimi K., Usta D. D., Çelikbıçak Ö., Pinar A., ​​Salih B., Tuncel A. (2017). Ti (IV) carrying polydopamine-coated, monodisperse-porous SiO2 microspheres with stable magnetic properties for highly selective enrichment of phosphopeptides. Colloids and Surfaces B: Biointerfaces, 153, 280-290.

• Kip Ç., Demir C., Tuncel A. (2017). One pot synthesis of carboxyl functionalized-polyhedral oligomeric siloxane based monolith via photoinitiated thiol-methacrylate polymerization for nano-hydrophilic interaction chromatography. Journal of Chromatography A, 1502, 14-23.

• Günal G., Kip Ç., Öğüt S. E., Usta D. D., Şenlik E., Kibar G., Tuncel A. (2017). Human genomic DNA isolation from whole blood using a simple microfluidic system with silica-and polymer-based stationary phases. Materials Science and Engineering: C, 74, 10-20.

• Hamaloğlu K. O., Sağ E., Tuncel A. (2017). Bare, gold and silver nanoparticle decorated, monodisperse-porous titania microbeads for photocatalytic dye degradation in a newly constructed microfluidic, photocatalytic packed-bed reactor. Journal of Photochemistry and Photobiology A: Chemistry, 332, 60-65.

• Salimi K., Usta D. D., Çelikbıçak Ö., Pınar A., ​​Salih B., Tuncel A. (2017). Highly selective enrichment of phosphopeptides by titanium (IV) attached monodisperse-porous poly (vinylphosphonic acid-co-ethylene dimethacrylate) microspheres. Journal of Chromatography A, 1496, 9-19.

• Kip Ç., Tuncel A. (2017). HILIC stationary phase based on monodisperse-porous polymethacrylate beads functionalized with zwitterionic molecular brushes. Chromatographia, 80 (4), 565-575.


B. International Congresses
• Gökcal B., Kip C., Tuncel A. Colorimetric method for ovalbumin detection using a new magnetic nanozyme. International Eurasian Conference on Biotechnology and Biochemistry, 2020.

• Yıldırım D., Kip C., Tuncel A. A novel nanozyme for colorimetric detection of L-histidine. VI. International Conference on New Trends in Chemistry, 2020.
• Tosun R., Hamaloğlu K. Ö., Tuncel A. Iridium oxide immobilized on cerium dioxide for enhanced water oxidation. VI. International Conference on New Trends in Chemistry, 2020.

• Ogut E., Kip C., Gökcal., Yıldırım D., Tuncel A. A new group of nanozymes for detection of some common biomolecules. HPLC 2019 - 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2019, Milan, Italy.

• Gökçal B., Kip Ç., Tosun R., Demir C., Tuncel A. Effects of process conditions on peroxidase-like activity of monodisperse-porous metal oxide microspheres modified with cupric oxide. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Gökçal B., Demir C., Tuncel A. Peroxidase-like activity of magnetic, monodisperse-porous SiO2 microspheres for sensing of proteins. 32. International Symposium on Chromatography 2018, Cannes, France.

• Kip Ç., Gökçal B., Demir C., Tuncel A. Effects of vitamins on the peroxidase like activity of monodisperse-porous MnO2 microspheres 5 μm in size. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Demir C., Tuncel A. Development of a new early stage tumor marker using molecular imprinting 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip F. Ç., Koçer İ., Çambay F., Çelik E., Tuncel A. A microfluidic system based on an organosilicon monolith for affinity separation of histidine-tagged proteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Süngü Ç., Kip Ç., Tuncel A. Boronate affinity separation of β-NAD via molecularly imprinted silica particles in batch and microfluidic systems. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Yıldırım D., Süngü Ç.Z., Tuncel A. Microfluidic system for immobilized metal affinity separation of phosphoproteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Alan B., Kip Ç., Tuncel A. Molecularly imprinted monodisperse-porous, silica microbeads for folic acid determination. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Alpaslan S., Tosun Babacan R., Kip C., Alan B., Tuncel A. Titania based immobilized metal affinity sorbent for the purification of histidine-rich proteins. 32. International Symposium on Chromatography, 2018, Cannes, France.

• Kip Ç., Demir M.C., Tuncel A. Hybrid stationary phases with retention independent separation performance in nano-hydrophilic interaction chromatography. HPLC 2017-45. International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Demir M.C., Nedjip G., Tuncel A. Preparation of phenylboronic acid functionalized-polyhedral oligomeric siloxane monolithic column via one-pot approach for nano liquid chromatography of small molecules. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Seperations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Demir M.C., Kuban A., Tuncel A. Alkanethiol functionalized organosilicon monoliths via one-pot thiol-methacrylate polymerization for nano-reversed phase chromatography. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Usta DD, Kip Ç., Salimi K., Celikbıçak Ö., Pınar A., ​​Salih B., Tuncel A. Phosphopeptide enrichment from human-serum via immobilized metal affinity chromatography using phosphonic acid functionalized capillary monolith as a stationary phase in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Durmaz M, Salimi K., Kuban A., Tuncel A. Phosphopeptide enrichment from human-serum via immobilized metal affinity chromatography using phosphonic acid functionalized capillary monolith as a stationary phase in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Kip Ç., Nedjip G., Demir M.C., Tuncel A. Preparation of phenylboronic acid functionalized hybrid monolithic column for boronate affinity chromatography of diol compounds in a microfluidic system. HPLC 2017 - 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017, Prague, Czech Republic.

• Salimi K., Usta D.D., Çelikbıçak Ö., Salih B., Tuncel A., Phosphopeptide enrichment using Ti (IV) attached monodisperse-porous poly (phosphonic acid) based microspheres. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Usta D.D., Salimi K., Kip Ç., Çelikkaya B., Tuncel A. Polydopamine coated, magnetic monodisperse-porous silica microspheres as a stationary phase for protein separation by immobilized metal affinity chromatography (IMAC). 44. International Symposium on High Performance Liquid Separations and Related Techniques, June 19-24th, 2016, San Fransisco, U.S.A.

• Kip Ç., Usta D.D., Salimi K., Gülüşür H., Tuncel A. Batch and microfluidic isolation of diol carrying biomolecules by a boronate affinity sorbent based on monodisperse-porous microspheres. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Usta D.D., Salimi K., Koçer İ., Çelik E., Tuncel A. A new magnetic-silica based sorbent for iolation of green fluorescent protein by immobilized metal affinity chromatography. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Kip Ç., Usta D.D., İpek Ö., Demir M.C., Tuncel A. Immobilized metal affinity microchromatography using phosphonic acid functionalized polymethacrylate based monolithic stationary phases carrying lantanide ions. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Demir M.C., Kip Ç., Tuncel A. Nano-HILIC applications of polyhedral oligomeric silsesquioxane based capillary monoliths functionalized with anionic and cationic ligands. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

• Sağ E., Hamaloğlu K.Ö., Demir M.C., Tuncel A. Ti (IV) functionalized monodisperse titania microspheres for nucleoside isolation by immobilized metal affinity chromatography. 44. International Symposium on High Performance Liquid Separations and Related Techniques, 2016, San Fransisco, U.S.A.

Links