Cell Electrophysiology and Imaging Laboratory

About

Research Infrastructure

•   Voltage-clamp
•   Patch clamp systems
•   Confocal microscope
•   Cell culture system
•   Molecular biology system

Researchers

•    Prof. Dr. Nuhan Puralı
•    Arş. Gör. Berk Sağlam
•    Arş. Gör. Nazlı Coşkun

Ongoing Projects

•  218S553, Determination of Structural and Functional Characteristics of Proteins Encoded by Genes Cloned in Relation to Mechano-Electrical Cycle in a Primary Receptor Neuron, 1001 - Research, Executive, Enforcement, ARDEB, SBAG - Health Sciences Research Support Group, Dates of Joining/Leaving the Project: 15.04. 2019 - 15.04.2022, Project Start/End Dates: 15.04.2019 - 15.04.2022.

Completed Projects

 •   TUBITAK1. 115R256, Hacettepe University R&D Strategy Document (Molecular Medicine), 1000 - University Research Potential, Executive, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Project Join/Leave Dates: 01.07.2016 - 01.01.2017, Project Start /End Dates: 01.07.2016 - 01.01.2017.
•   214S174, Investigation of the Effect of Desmin Mutation on Protein Function in Zebrafish Model, 1001 - Research, Researcher/Specialist, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Joining/Leaving the Project Dates: 15.04.2015 - 15.10.2018, Project Start /End Dates: 15.04.2015 - 15.10.2018.
•    215S995, Development of New Adjuvant/Carrier Systems to Increase Immunogenicity of Subunit Protein Antigens, International, Researcher/Specialist, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Project Join/Leave Dates: 01.02.2017 - 01.02.2019, Project Start/End Dates: 01.02.2017 - 01.02.2019.
•   Project Code: 014 D 08 101 006-722 Project Type: Support Baş. Date: 22.08.2014 End Date: 18.09.2015 Position: Executive Duration: 10(months) Title: Crayfish (Astacu s lepto dactylu s) cloning of sodium-calcium counter-exchanger (NCX) gene and determination of its expression level in different tissues
•   Project Code: TB B -2017-16192 Project Type: B. Start Date of Success: 04.10.2017 End Date: 28.09.2018 Position: Executive Term: 12(months) Title: Project for Participation in 11 FENS Forum Berlin Meeting
•   Project Code: TED -2017-15468 Project Type: Event Participation Baş. Date: 13.07.2017 End Date: 22.01.2018 Position: Researcher Duration: 6(months)Title: The Effect of Ultrasonic Activation on the Penetration of Calcium Silicate Based Cements in Teeth with Open Apex
•   Project Code: TSA-2017-15403 Project Type: K. Research Start Date: 21.11.2017 End Date: 28.01.2020 Task: Executive Duration: 24(months) Title: Cloning of genes belonging to structures responsible for mechanoelectrical cycle in a primary receptor neuron and determination of molecular properties of encoded proteins
•   Project Code: THD -2016-12898 Project Type: Rapid Support Start Date: 14.11.2016 End Date: 28.06.2017 Position: Executive Duration: 6(months) Title: Cloning Vector Synthesis for Sodium Calcium Exchanger Gene KT697616
•   Project Code: THD -2015-8022 Project Type: Rapid Support Start Date: 12.10.2015 End Date: 24.10.2016 Position: Executive Duration: 12(months) Title: Plasmid for crayfish sodium channel gene (834104 PR 1 KT187402) production.
•   Project Code: THD -2015-7717 Project Type: Rapid Support Start Date: 27.08.2015 End Date: 28.04.2017 Position: Researcher Duration: 12(months) Title: Investigation of Mitochondrial Dynamics in Megaconial Congenital Muscular Dystrophy Disease

Publications

•    Development and in vitro evaluation of a new adjuvant system containing Salmonella Typhiporins and chitosan By: Yuksel, Selin; Pekcan, Mert; Purali, Nuhan; et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS Volume: ‏ 57 Article Number: 119129 Published: ‏MAR 30 2020
•    Dentinal Tubule Penetration and Retreatability of a Calcium Silicate-based Sealer Tested in Bulkor with Different Main Core Material By: Eymirli, Ayhan; Sungur, Derya Deniz; Uyanik, Ozgur; et al. JOURNAL OF ENDODONTICS Volume: ‏ 45Issue: ‏8 Pages: ‏ 1036-1040Published: AUG 2019
•    Effect of ultrasonic activation on dentinal tubule penetration of calcium silicate-based cements By: Aksel, Hacer; Arslan, Elifcan; Purali, Nuhan; et al. MICROSCOPY RESEARCH AND TECHNIQUE Volume: ‏ 8Issue: ‏ 5Pages: ‏ 624-62 Published: ‏ MAY 2019
•    Modeling of a unique desmin mutation in zebra fish by using genome editing brings new insights into desmin function By: Kurekci, G. Kayman; Koyunlar, C.; Kural, E.; et al. Conference: 50th European-Society-of-Human-Genetics (ESHG) Conference Location: ‏ Copenhagen, DENMARK Date: ‏ MAY 27-30, 2017 Sponsor(s): ‏European Soc Human Genet EUROPEAN JOURNAL OF HUMAN GENETICS Volume: ‏ 26Supplement: S Pages: ‏ 427-427Meeting Abstract: ‏ P10.14B Published: ‏ OCT 2018
•    Analysing the expression profiles of human DES orthologousdesma and desmb by using knockout zebrafish models By: Koyunlar, C.; Kayman-Kurekci, G.; Kural, E.; et al. Conference: 50th European-Society-of-Human-Genetics (ESHG) Conference Location: ‏ Copenhagen, DENMARK Date: ‏ MAY 27-30, 2017 Sponsor(s): ‏EuropeanSoc Human Genet EUROPEAN JOURNAL OF HUMAN GENETICS Volume: ‏ 26  Supplement: ‏ S Pages: ‏ 618-618Meeting Abstract: ‏ P13.12D  Published: ‏ OCT 2018
•    Cloning of a putative sodium/calcium exchanger gene in the crayfish By: Ergin, Bora; Purali, NuhanINVERTEBRATE NEUROSCIENCE Volume: ‏ 18  Issue: ‏3 Article Number: 9 Published: ‏ SEP 2018
•    Recombinant amelogenin regulates the bioactivity of Mouse cementoblasts in vitro By: Hakki, Sema S.; Bozkurt, S. Buket; Tuerkay, Emre; et al. INTERNATIONAL JOURNAL OF ORAL SCIENCE Volume: ‏ 10 Pages: ‏ 1-10 Article Number: 15 Published: ‏ MAY 9 2018
•    Potential role of pyrin, the protein mutated in familial Mediterranean fever, during inflammatory cell migration By: Balci-Peynircioglu, B.; Akkaya-Ulum, Y. Z.; Avci, E.; et al. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY Volume: ‏ 36Issue: ‏ 6Supplement: ‏ 115Pages: S116-S124 Published: ‏ 2018
•    Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis By: Aksel, Hacer; Eren, Selen Kucukkaya; Purali, Nuhan; et al. MICROSCOPY RESEARCH AND TECHNIQUE Volume: ‏80 Issue: ‏ 12 Pages: ‏ 1323-1327 Published: ‏ DEC 2017
•    Fast calcium transients translate the distribution and conduction of neural activity in different regions of a single sensory neuron By: Purali, Nuhan INVERTEBRATE NEUROSCIENCE Volume: ‏ 17Issue: ‏ 3 Article Number: 7 Published: ‏ SEP 2017
•    Effect of a Low SurfaceTension Vehicle on the Dentinal Tubule Penetration of Calcium Hydroxide and Triple Antibiotic Paste (vol 43, pg 452, 2017) By: Sungur, Derya Deniz; Aksel, Hacer; Purali, NuhanJOURNAL OF ENDODONTICS  Volume: ‏ 43 Issue: 7 Pages: ‏ 1213-1213Published: ‏ JUL 2017
•    Effect of a Low Surface Tension Vehicle on the Dentinal Tubule Penetration of Calcium Hydroxideand Triple Antibiotic Paste By: Sungur, Derya Deniz; Aksel, Hacer; Purali, Nuhan JOURNAL OF ENDODONTICS Volume: 43 Issue: ‏3 Pages: ‏ 452-455 Published: ‏ MAR 2017
•    Cloning and molecular characterization of a putative voltage-gate dsodium channel gene in the crayfish By: Coskun, Cagil; Purali, Nuhan INVERTEBRATE NEUROSCIENCE  Volume: 16 Issue: 2 ArticleNumber: 2 Published: ‏JUN 2016
•    Comparison of the efficiency and relevancy of reference genes in crayfish tissue samples By: Ergin, Bora; Purali, NuhanTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI  Volume: ‏ 4 Issue: ‏ 3Pages: ‏ 144-149  Published: ‏ JUN 2016

Links

About

Research Infrastructure

•   Voltage-clamp
•   Patch clamp systems
•   Confocal microscope
•   Cell culture system
•   Molecular biology system

Researchers

•    Prof. Dr. Nuhan Puralı
•    Arş. Gör. Berk Sağlam
•    Arş. Gör. Nazlı Coşkun

Ongoing Projects

•  218S553, Determination of Structural and Functional Characteristics of Proteins Encoded by Genes Cloned in Relation to Mechano-Electrical Cycle in a Primary Receptor Neuron, 1001 - Research, Executive, Enforcement, ARDEB, SBAG - Health Sciences Research Support Group, Dates of Joining/Leaving the Project: 15.04. 2019 - 15.04.2022, Project Start/End Dates: 15.04.2019 - 15.04.2022.

Completed Projects

 •   TUBITAK1. 115R256, Hacettepe University R&D Strategy Document (Molecular Medicine), 1000 - University Research Potential, Executive, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Project Join/Leave Dates: 01.07.2016 - 01.01.2017, Project Start /End Dates: 01.07.2016 - 01.01.2017.
•   214S174, Investigation of the Effect of Desmin Mutation on Protein Function in Zebrafish Model, 1001 - Research, Researcher/Specialist, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Joining/Leaving the Project Dates: 15.04.2015 - 15.10.2018, Project Start /End Dates: 15.04.2015 - 15.10.2018.
•    215S995, Development of New Adjuvant/Carrier Systems to Increase Immunogenicity of Subunit Protein Antigens, International, Researcher/Specialist, Concluded, ARDEB, SBAG - Health Sciences Research Support Group, Project Join/Leave Dates: 01.02.2017 - 01.02.2019, Project Start/End Dates: 01.02.2017 - 01.02.2019.
•   Project Code: 014 D 08 101 006-722 Project Type: Support Baş. Date: 22.08.2014 End Date: 18.09.2015 Position: Executive Duration: 10(months) Title: Crayfish (Astacu s lepto dactylu s) cloning of sodium-calcium counter-exchanger (NCX) gene and determination of its expression level in different tissues
•   Project Code: TB B -2017-16192 Project Type: B. Start Date of Success: 04.10.2017 End Date: 28.09.2018 Position: Executive Term: 12(months) Title: Project for Participation in 11 FENS Forum Berlin Meeting
•   Project Code: TED -2017-15468 Project Type: Event Participation Baş. Date: 13.07.2017 End Date: 22.01.2018 Position: Researcher Duration: 6(months)Title: The Effect of Ultrasonic Activation on the Penetration of Calcium Silicate Based Cements in Teeth with Open Apex
•   Project Code: TSA-2017-15403 Project Type: K. Research Start Date: 21.11.2017 End Date: 28.01.2020 Task: Executive Duration: 24(months) Title: Cloning of genes belonging to structures responsible for mechanoelectrical cycle in a primary receptor neuron and determination of molecular properties of encoded proteins
•   Project Code: THD -2016-12898 Project Type: Rapid Support Start Date: 14.11.2016 End Date: 28.06.2017 Position: Executive Duration: 6(months) Title: Cloning Vector Synthesis for Sodium Calcium Exchanger Gene KT697616
•   Project Code: THD -2015-8022 Project Type: Rapid Support Start Date: 12.10.2015 End Date: 24.10.2016 Position: Executive Duration: 12(months) Title: Plasmid for crayfish sodium channel gene (834104 PR 1 KT187402) production.
•   Project Code: THD -2015-7717 Project Type: Rapid Support Start Date: 27.08.2015 End Date: 28.04.2017 Position: Researcher Duration: 12(months) Title: Investigation of Mitochondrial Dynamics in Megaconial Congenital Muscular Dystrophy Disease

Publications

•    Development and in vitro evaluation of a new adjuvant system containing Salmonella Typhiporins and chitosan By: Yuksel, Selin; Pekcan, Mert; Purali, Nuhan; et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS Volume: ‏ 57 Article Number: 119129 Published: ‏MAR 30 2020
•    Dentinal Tubule Penetration and Retreatability of a Calcium Silicate-based Sealer Tested in Bulkor with Different Main Core Material By: Eymirli, Ayhan; Sungur, Derya Deniz; Uyanik, Ozgur; et al. JOURNAL OF ENDODONTICS Volume: ‏ 45Issue: ‏8 Pages: ‏ 1036-1040Published: AUG 2019
•    Effect of ultrasonic activation on dentinal tubule penetration of calcium silicate-based cements By: Aksel, Hacer; Arslan, Elifcan; Purali, Nuhan; et al. MICROSCOPY RESEARCH AND TECHNIQUE Volume: ‏ 8Issue: ‏ 5Pages: ‏ 624-62 Published: ‏ MAY 2019
•    Modeling of a unique desmin mutation in zebra fish by using genome editing brings new insights into desmin function By: Kurekci, G. Kayman; Koyunlar, C.; Kural, E.; et al. Conference: 50th European-Society-of-Human-Genetics (ESHG) Conference Location: ‏ Copenhagen, DENMARK Date: ‏ MAY 27-30, 2017 Sponsor(s): ‏European Soc Human Genet EUROPEAN JOURNAL OF HUMAN GENETICS Volume: ‏ 26Supplement: S Pages: ‏ 427-427Meeting Abstract: ‏ P10.14B Published: ‏ OCT 2018
•    Analysing the expression profiles of human DES orthologousdesma and desmb by using knockout zebrafish models By: Koyunlar, C.; Kayman-Kurekci, G.; Kural, E.; et al. Conference: 50th European-Society-of-Human-Genetics (ESHG) Conference Location: ‏ Copenhagen, DENMARK Date: ‏ MAY 27-30, 2017 Sponsor(s): ‏EuropeanSoc Human Genet EUROPEAN JOURNAL OF HUMAN GENETICS Volume: ‏ 26  Supplement: ‏ S Pages: ‏ 618-618Meeting Abstract: ‏ P13.12D  Published: ‏ OCT 2018
•    Cloning of a putative sodium/calcium exchanger gene in the crayfish By: Ergin, Bora; Purali, NuhanINVERTEBRATE NEUROSCIENCE Volume: ‏ 18  Issue: ‏3 Article Number: 9 Published: ‏ SEP 2018
•    Recombinant amelogenin regulates the bioactivity of Mouse cementoblasts in vitro By: Hakki, Sema S.; Bozkurt, S. Buket; Tuerkay, Emre; et al. INTERNATIONAL JOURNAL OF ORAL SCIENCE Volume: ‏ 10 Pages: ‏ 1-10 Article Number: 15 Published: ‏ MAY 9 2018
•    Potential role of pyrin, the protein mutated in familial Mediterranean fever, during inflammatory cell migration By: Balci-Peynircioglu, B.; Akkaya-Ulum, Y. Z.; Avci, E.; et al. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY Volume: ‏ 36Issue: ‏ 6Supplement: ‏ 115Pages: S116-S124 Published: ‏ 2018
•    Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis By: Aksel, Hacer; Eren, Selen Kucukkaya; Purali, Nuhan; et al. MICROSCOPY RESEARCH AND TECHNIQUE Volume: ‏80 Issue: ‏ 12 Pages: ‏ 1323-1327 Published: ‏ DEC 2017
•    Fast calcium transients translate the distribution and conduction of neural activity in different regions of a single sensory neuron By: Purali, Nuhan INVERTEBRATE NEUROSCIENCE Volume: ‏ 17Issue: ‏ 3 Article Number: 7 Published: ‏ SEP 2017
•    Effect of a Low SurfaceTension Vehicle on the Dentinal Tubule Penetration of Calcium Hydroxide and Triple Antibiotic Paste (vol 43, pg 452, 2017) By: Sungur, Derya Deniz; Aksel, Hacer; Purali, NuhanJOURNAL OF ENDODONTICS  Volume: ‏ 43 Issue: 7 Pages: ‏ 1213-1213Published: ‏ JUL 2017
•    Effect of a Low Surface Tension Vehicle on the Dentinal Tubule Penetration of Calcium Hydroxideand Triple Antibiotic Paste By: Sungur, Derya Deniz; Aksel, Hacer; Purali, Nuhan JOURNAL OF ENDODONTICS Volume: 43 Issue: ‏3 Pages: ‏ 452-455 Published: ‏ MAR 2017
•    Cloning and molecular characterization of a putative voltage-gate dsodium channel gene in the crayfish By: Coskun, Cagil; Purali, Nuhan INVERTEBRATE NEUROSCIENCE  Volume: 16 Issue: 2 ArticleNumber: 2 Published: ‏JUN 2016
•    Comparison of the efficiency and relevancy of reference genes in crayfish tissue samples By: Ergin, Bora; Purali, NuhanTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI  Volume: ‏ 4 Issue: ‏ 3Pages: ‏ 144-149  Published: ‏ JUN 2016

Links