Cell Physiology Laboratory

About

Research Infrastructure

• Laminar Cabinet, Class II Type (Biolab, Faster, BH 2004)
• ELISA plate reader (Biochrom, EZ Read 400, Cambridge)
• Water Bath 14 Lt Digital GFL (1003)
• CO2 Incubator (Heracell 150)
• Inverted microscope with fluorescent attachment (Olympus IX70 Inverted Microscope, Japan)
• Centrifuge (Hettich Universal 32)
• Microtome (Leica 820, Jung Histocut)
• Oven (Thermal, G11420SD)
• Ultra pure water device (Nanopure Diamond, Barnstead)
• Distilled water device (MP, Minipure)
• Centrifuge mini (IsoLAB, GmbH)
• Gel Analyzer
• Thermal Cycler
• Fluorescent microscope with camera attachment
• Light microscope

Researchers

•    Prof. Dr. Mehmet Ali Onur
•    Doç. Dr. Ö. Aylin Gürpınar
•    Dr. Öğr. Üyesi Esin Çetin
•    Dr. Öğr. Üyesi Handan Sevim
•    Araş. Gör. Dr. Hanife Güler Tanır  

Ongoing Projects

• H.U. BAP PROJECT: ID: 18650 Project Code: FHD-2020-18650 Project Type: Quick Support Project Name: In vitro investigation of photoprotective effects of different bee venom components against UVA and UVB rays
• H.U. BAP PROJECT: ID: 18273 Project Code: FYL-2019-18273 Project Type: M.Sc. Support Project Name: Comparison of the protective effects of alpha-tocopherol and alpha-tocotrienol forms of vitamin E against beta amyloid plaque accumulation in the brain
• FHD-2020-18482, Investigation of antimicrobial and antibiofilm effects and cytotoxic potentials of plant-containing hydrogel and microbial celluloses, 2020-2022
• FUI-2020-18907, Investigation of oncogenic effects of HPV8 E6E7 proteins, 2020-2021
• Investigation of Aebp1 / Aclp Gene Function, TÜBİTAK, 119S521, National.
• Demonstration of the Immune Modulatory Effect of Mesenchymal Stem Cells in Hemophagocytic Syndrome Patient Samples, TÜBİTAK, 219S897, National.

Completed Projects

• H.U. BAP PROJECT: ID: 885 Project Code: 013 01 601 002 Project type: Comprehensive Research Support Project Name: Ectopic migration of differentiated marked cells originating from mesenchymal stem cells
• H.U. BAP PROJECT: ID: 11684 Project Code: FHD-2016-11684 Project Type: Quick Support Support Project Name: The Effect of Honey Bee Venom on Stem Cells of Adipose Tissue Origin
• H.U. BAP PROJECT: ID: 7428 Project Code: FDK-2015-7428 Project Type: Ph.D.Support Project Name: Investigation of In vivo Post-Implantation Ectopic Migration Potential of Marked Cardiomyocytes Differentiated from Mesenchymal Stem Cells
• THD-2019-18131, Effect of Human Papilloma Virus Infection on Wnt/Beta-Catenin Signaling Pathway, Autophagy and Apoptosis, 2019-2020
• THD-2018-17493, Investigation of cell morphology and physiology in buccal smear samples from term and preterm newborns Wntbetakatenin signaling pathway and apoptosis, 2018-2020
• Preparation and characterization of FHD-2018-17008, Dextran-9Z 11E Conjugated Linoleic Acid conjugate and investigation of the effect of Wntbetakatenin on signaling pathway and apoptosis in different cancer cells, 2018-2019
• Development of a cell line model with perforin defect as a basis for familial hemophagocytic lymphohistiocytosis research, Hacettepe University Scientific Research Unit, project no. 013 D12 601 001, 2013-2015, Assistant researcher, National.
• Investigation of Immune Modulatory Effects of Mesenchymal Stem Cells in an In Vitro Model to Form the Basis for Clinical Applications of Hemophagocytic Syndrome, TÜBİTAK, Project No. 114S057, National.
• Investigation of Notch Signaling Pathway in Human Adipose Tissue Mesenchymal Stem Cells, Hacettepe University Scientific Research Unit, project number FHD-2015-5324, National.
• Investigation of the effect of fungal-derived Bassiatin (3r, 6r) on MCF7 and Hmec cell lines, TÜBİTAK, project 215S096, 29500TL, 01.01.2016-21.02.2017, Assistant researcher, National.

Publications

• Beksac MS, Donmez HG. Impact of hydroxychloroquine on the gestational outcomes of pregnant women with immune system problems that necessitate the use of the drug. J Obstet Gynaecol Res. 2020. doi:10.1111/jog.14561
• Donmez HG. β-Catenin immunocytochemical reactivity in cervicovaginal smears during regular menstrual cycles. Asian Biomedicine 2020;14(5):187-194. doi:10.1515/abm-2020-0027
• Donmez HG, Sahal G, Akgor U, Cagan M, Ozgul N, Beksac MS. The relationship between the presence of HPV infection and biofilm formation in cervicovaginal smears. Infection 2020 Oct;48(5):735-740. doi: 10.1007/s15010-020-01478-5.
• Akbay, E., Sevim, H., Gurpinar, O.A., Günaydın, S., Onur, M.A. Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model, Hacettepe Journal of Biology and Chemistry, 2016, , 44 [3], 283–292.
• Atalay Ö, Sevim H, Karahisar Turan S, Onur M A, Gürpınar Ö A. The effects of mesenchymal stem cells on the structure and contractile force of the carotid artery in a rat aneurysm model. Turk J Med Sci. 2017;47(5):1634-1641. doi: 10.3906/sag-1701-183.
• Günaydın Y, Sevim H, Tanyolaç D, Gürpınar Ö A. Ozonated Olive Oil with a High Peroxide Value for Topical Applications: In-Vitro Cytotoxicity Analysis with L929 Cells. Ozone: Science & Engineering, 2018, 40.1: 37-43.
• Akay C, Tanış MÇ, Sevim H. Effect of artificial saliva with different pH levels on the cytotoxicity of soft denture lining materials. Int J Artif Organs. 2017;40(10):581-588. doi: 10.5301/ijao.5000614. Epub 2017 Jun 27.
• Sevim, H., Akbay, E., Gürpınar, ÖA., Günaydın, S., Onur, MA. Cardiomyogenic Gene Expression Profile of 5-Azacytidine-Treated Rat Bone Marrow Mesenchymal Stem Cells. Hacettepe Journal of Biology and Chemistry, 2017, 45 (3), 343–350.
• Sevim H, Kocaefe YÇ, Onur MA, Uçkan-Çetinkaya D, Gürpınar OA. Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Stem Cell Res Ther. 2018;9(1):198.
• Çakırbay Tanış M, Akay C, Sevim H. Cytotoxicity of long-term denture base materials. Int J Artif Organs. int. 2018;41(10):677-683. doi: 10.1177/0391398818786884.
• Akbay, E., Özenilir, Ç., Gençay Çelemli, Ö., Durukan, A. B., Onur, M. A., Sorkun, K., “Effects of poropolis on warfarin efficacy”, Kardiochirugia i Torakochirurgia Polska 2017; 14 (1): 43-46.
• Akbay, E., Onur, M.A., Investigation of Survival and Migration Potential of Differentiated Cardiomyocytes Transplanted With Decellularized Heart Scaffold. Journal of Biomedical Materials Research Part A 2018; 9999:1-10
• Sesli, M., Akbay, E., Onur, M.A., Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods. Turkish Journal of Biology 2018; 42.
• Durukan, A.B., Akbay, E., Ünlü, A., Özdemir, A., Onur, M.A., The cytotoxic properties and apoptotic potential of N-butyl and 2-octyl cyanoacrylates used in surgical treatment of chronic venous insufficiency. Turkish Journal of Thoracic and Cardiovascular Surgery, 2019;27(2):185-19.
• Akbay, E., Erdem, B., Ünlü, A., Durukan, A. B., Onur, M. A. Effects of N-acetyl cysteine, vitamin E and vitamin C on liver glutathione levels following amiodarone treatment in rats. Kardiochiurgia i torakochirurgia polska= Polish journal of cardio-thoracic surgery, 2019; 16(2), 88.
• Kip, Ç., Akbay, E., Gökçal, B., Okyay Savaş, B., Onur, MA, Tuncel, A., Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached -silica microspheres containing accessible magnetite nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020; 124812.

Links

About

Research Infrastructure

• Laminar Cabinet, Class II Type (Biolab, Faster, BH 2004)
• ELISA plate reader (Biochrom, EZ Read 400, Cambridge)
• Water Bath 14 Lt Digital GFL (1003)
• CO2 Incubator (Heracell 150)
• Inverted microscope with fluorescent attachment (Olympus IX70 Inverted Microscope, Japan)
• Centrifuge (Hettich Universal 32)
• Microtome (Leica 820, Jung Histocut)
• Oven (Thermal, G11420SD)
• Ultra pure water device (Nanopure Diamond, Barnstead)
• Distilled water device (MP, Minipure)
• Centrifuge mini (IsoLAB, GmbH)
• Gel Analyzer
• Thermal Cycler
• Fluorescent microscope with camera attachment
• Light microscope

Researchers

•    Prof. Dr. Mehmet Ali Onur
•    Doç. Dr. Ö. Aylin Gürpınar
•    Dr. Öğr. Üyesi Esin Çetin
•    Dr. Öğr. Üyesi Handan Sevim
•    Araş. Gör. Dr. Hanife Güler Tanır  

Ongoing Projects

• H.U. BAP PROJECT: ID: 18650 Project Code: FHD-2020-18650 Project Type: Quick Support Project Name: In vitro investigation of photoprotective effects of different bee venom components against UVA and UVB rays
• H.U. BAP PROJECT: ID: 18273 Project Code: FYL-2019-18273 Project Type: M.Sc. Support Project Name: Comparison of the protective effects of alpha-tocopherol and alpha-tocotrienol forms of vitamin E against beta amyloid plaque accumulation in the brain
• FHD-2020-18482, Investigation of antimicrobial and antibiofilm effects and cytotoxic potentials of plant-containing hydrogel and microbial celluloses, 2020-2022
• FUI-2020-18907, Investigation of oncogenic effects of HPV8 E6E7 proteins, 2020-2021
• Investigation of Aebp1 / Aclp Gene Function, TÜBİTAK, 119S521, National.
• Demonstration of the Immune Modulatory Effect of Mesenchymal Stem Cells in Hemophagocytic Syndrome Patient Samples, TÜBİTAK, 219S897, National.

Completed Projects

• H.U. BAP PROJECT: ID: 885 Project Code: 013 01 601 002 Project type: Comprehensive Research Support Project Name: Ectopic migration of differentiated marked cells originating from mesenchymal stem cells
• H.U. BAP PROJECT: ID: 11684 Project Code: FHD-2016-11684 Project Type: Quick Support Support Project Name: The Effect of Honey Bee Venom on Stem Cells of Adipose Tissue Origin
• H.U. BAP PROJECT: ID: 7428 Project Code: FDK-2015-7428 Project Type: Ph.D.Support Project Name: Investigation of In vivo Post-Implantation Ectopic Migration Potential of Marked Cardiomyocytes Differentiated from Mesenchymal Stem Cells
• THD-2019-18131, Effect of Human Papilloma Virus Infection on Wnt/Beta-Catenin Signaling Pathway, Autophagy and Apoptosis, 2019-2020
• THD-2018-17493, Investigation of cell morphology and physiology in buccal smear samples from term and preterm newborns Wntbetakatenin signaling pathway and apoptosis, 2018-2020
• Preparation and characterization of FHD-2018-17008, Dextran-9Z 11E Conjugated Linoleic Acid conjugate and investigation of the effect of Wntbetakatenin on signaling pathway and apoptosis in different cancer cells, 2018-2019
• Development of a cell line model with perforin defect as a basis for familial hemophagocytic lymphohistiocytosis research, Hacettepe University Scientific Research Unit, project no. 013 D12 601 001, 2013-2015, Assistant researcher, National.
• Investigation of Immune Modulatory Effects of Mesenchymal Stem Cells in an In Vitro Model to Form the Basis for Clinical Applications of Hemophagocytic Syndrome, TÜBİTAK, Project No. 114S057, National.
• Investigation of Notch Signaling Pathway in Human Adipose Tissue Mesenchymal Stem Cells, Hacettepe University Scientific Research Unit, project number FHD-2015-5324, National.
• Investigation of the effect of fungal-derived Bassiatin (3r, 6r) on MCF7 and Hmec cell lines, TÜBİTAK, project 215S096, 29500TL, 01.01.2016-21.02.2017, Assistant researcher, National.

Publications

• Beksac MS, Donmez HG. Impact of hydroxychloroquine on the gestational outcomes of pregnant women with immune system problems that necessitate the use of the drug. J Obstet Gynaecol Res. 2020. doi:10.1111/jog.14561
• Donmez HG. β-Catenin immunocytochemical reactivity in cervicovaginal smears during regular menstrual cycles. Asian Biomedicine 2020;14(5):187-194. doi:10.1515/abm-2020-0027
• Donmez HG, Sahal G, Akgor U, Cagan M, Ozgul N, Beksac MS. The relationship between the presence of HPV infection and biofilm formation in cervicovaginal smears. Infection 2020 Oct;48(5):735-740. doi: 10.1007/s15010-020-01478-5.
• Akbay, E., Sevim, H., Gurpinar, O.A., Günaydın, S., Onur, M.A. Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model, Hacettepe Journal of Biology and Chemistry, 2016, , 44 [3], 283–292.
• Atalay Ö, Sevim H, Karahisar Turan S, Onur M A, Gürpınar Ö A. The effects of mesenchymal stem cells on the structure and contractile force of the carotid artery in a rat aneurysm model. Turk J Med Sci. 2017;47(5):1634-1641. doi: 10.3906/sag-1701-183.
• Günaydın Y, Sevim H, Tanyolaç D, Gürpınar Ö A. Ozonated Olive Oil with a High Peroxide Value for Topical Applications: In-Vitro Cytotoxicity Analysis with L929 Cells. Ozone: Science & Engineering, 2018, 40.1: 37-43.
• Akay C, Tanış MÇ, Sevim H. Effect of artificial saliva with different pH levels on the cytotoxicity of soft denture lining materials. Int J Artif Organs. 2017;40(10):581-588. doi: 10.5301/ijao.5000614. Epub 2017 Jun 27.
• Sevim, H., Akbay, E., Gürpınar, ÖA., Günaydın, S., Onur, MA. Cardiomyogenic Gene Expression Profile of 5-Azacytidine-Treated Rat Bone Marrow Mesenchymal Stem Cells. Hacettepe Journal of Biology and Chemistry, 2017, 45 (3), 343–350.
• Sevim H, Kocaefe YÇ, Onur MA, Uçkan-Çetinkaya D, Gürpınar OA. Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Stem Cell Res Ther. 2018;9(1):198.
• Çakırbay Tanış M, Akay C, Sevim H. Cytotoxicity of long-term denture base materials. Int J Artif Organs. int. 2018;41(10):677-683. doi: 10.1177/0391398818786884.
• Akbay, E., Özenilir, Ç., Gençay Çelemli, Ö., Durukan, A. B., Onur, M. A., Sorkun, K., “Effects of poropolis on warfarin efficacy”, Kardiochirugia i Torakochirurgia Polska 2017; 14 (1): 43-46.
• Akbay, E., Onur, M.A., Investigation of Survival and Migration Potential of Differentiated Cardiomyocytes Transplanted With Decellularized Heart Scaffold. Journal of Biomedical Materials Research Part A 2018; 9999:1-10
• Sesli, M., Akbay, E., Onur, M.A., Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods. Turkish Journal of Biology 2018; 42.
• Durukan, A.B., Akbay, E., Ünlü, A., Özdemir, A., Onur, M.A., The cytotoxic properties and apoptotic potential of N-butyl and 2-octyl cyanoacrylates used in surgical treatment of chronic venous insufficiency. Turkish Journal of Thoracic and Cardiovascular Surgery, 2019;27(2):185-19.
• Akbay, E., Erdem, B., Ünlü, A., Durukan, A. B., Onur, M. A. Effects of N-acetyl cysteine, vitamin E and vitamin C on liver glutathione levels following amiodarone treatment in rats. Kardiochiurgia i torakochirurgia polska= Polish journal of cardio-thoracic surgery, 2019; 16(2), 88.
• Kip, Ç., Akbay, E., Gökçal, B., Okyay Savaş, B., Onur, MA, Tuncel, A., Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached -silica microspheres containing accessible magnetite nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020; 124812.

Links