Medical and Industrial Biotechnology Laboratory

About

Research Infrastructure

• Hot Water Bath (Memert Brand)
• Microscope (2 Pieces) (Leica Brand)
• Centrifuge (Eppendorf Brand 5417c Model)
• Refrigerator (AEG Brand)
• Vortex (Ika Brand Genius 3 Mode)
• Precision Balance (Ika Brand Rct Basic Model)
• Precision Balance (Yellowline Brand Mst Digital Model)
• pH Meter (Schott Brand)
• pH Meter (Consort Brand)
• Magnetic Stirrer with Heating

Researchers

•    Prof. Dr. Işıl Seyis Bilkay
•    Araş. Gör. Dr. Hande Avcıoğlu
•    Araş. Gör. Dr. Sinem Diken Gür
•    Araş. Gör. Dr. Sezen Bilen Özyürek
•    Araş. Gör. Dr. Gülcan Şahal
•    Meriç Birben
•    Cansu Sevim
•    Cansu Köten

Ongoing Projects

• Evaluation of Mycoremediation Potentials of Fungi in terms of Petroleum Biodegradation Biosorption and Biosurfactant Production, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2019-Continuing.)
• Determination of Glycoprotein with Surface Plasmon Nanosensor, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2019-Continuing.)
• Investigation of Antimicrobial and Antibiofilm Effects of Biosurfactants to be Produced with Industrial Wastes, TÜBİTAK 2209/A UNIVERSITY STUDENTS RESEARCH PROJECTS SUPPORT PROGRAM, 1919B012000647, (Continued).
• Investigation of antimicrobial and antibiofilm effects and cytotoxic potentials of hydrogels and microbial celluloses containing herbal substances, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2020-Continuing).

Completed Projects

• Investigation of Cyanide Biodegradation by Klebsiella Pneumoniae and Klebsiella Ornithinolytica Species, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2015.
• Coimmobilized Enzyme Design with Two Different Support Materials, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2015.
• Coimmobilized Enzyme Design for Wastewater Pretreatment, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Thesis Project (PhD), 2016.
• Investigation of petroleum biodegradation by bacteria isolated from crude oil, drilling mud and mud pit, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2016.
• Investigation of Biofilms Formed by Different Clinical Candida Strains on Various Materials and Different Herbal Substances that Have an Effect on These Biofilms, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, PhD Thesis Project, 2016.
• Investigation of Cyanide Biodegradation by Fungal Sources, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2017.
• Investigation of the biodegradation of microorganisms isolated from crude oil, drilling mud and mud pit and various hydrocarbons, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, PhD Thesis Project, 2017.
• Investigation of the effects of bacterial consortia adapted to oiled sludge pit on petroleum biodegradation, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, (2017-2018).
• Escherichia coli Determination by Surface Plasmon Resonance Biosensor, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2018.
• Investigation of Antibiofilm Effects of Polycaprolactone Nanofibers Containing Various Essential Oils on Different Candida Species, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2018.
• Investigation of Biosurfactant Production by Bacterial Strains to be Isolated from Oil Field, TÜBİTAK 2209/A UNIVERSITY STUDENTS RESEARCH PROJECTS SUPPORT PROGRAM, 1919B011900736, (2019-2020).
• Research on the Use of Cellulose in Cyanide and Dye Removal, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2020.

Publications

• Avcioglu, N.H., Sahal, G., Bilkay, I., Investigation of Biofilm Formation in Clinically Acquired Escherichia coli Strains, Hacettepe J. Biol. & Chem., (2015), 43 (1), 43–48,
• Avcioglu, N.H., Seyis Bilkay, I., Assessment of five clinical Klebsiella isolates in terms of antibiotic resistance and plasmid profiles, Turkish Journal of Biochemistry, (2015), 40 (6), 448-455,
• Bilen Ozyurek, S., Seyis Bilkay, I. Determination of resistance in Pseudomonas aeruginosa strains against beta lactam aminoglycoside and ciprofloxacin group antibiotics, Hacettepe Journal of Biology & Chemistry, (2015), 43, 59-67.
• Sahal G., Nasseri B., Bilkay Seyis I., Piskin E. “Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces Against Clinical P. mirabilis, C. glabrata and C. tropicalis Strains. ” J Appl Biomater Funct Mater., (2015), 13(4), 351-355.
• Sahal G., Bilkay Seyis I.; Multidrug resistance by biofilm-forming clinical strains of Proteus mirabilis., Asian Biomedicine (2015), 9(4), 535-541.
• Avcioglu, NH, Sahal, G., Bilkay, I., Antibiofilm Effects of Citrus limonum and Zingiber officinale oils on biofilm formation of Klebsiella ornithinolytica, Klebsiella oxytoca and Klebsiella terrigena species, African Journal of Traditional, Complementary and Alternative Medicines, (2016) ), 13(6):61-67.
• Avcioglu, N.H., Seyis Bilkay, I., Biological Treatment of Cyanide by Using Klebsiella pneumoniae Species, Food Technology and Biotechnology, (2016), 54 (4), 450-454.
• Sahal, G., Avcioglu, NH, Seyis Bilkay, I., Higher Biofilm Formation by Multi-Drug Resistant K. pneumoniae and K. rhinoscleromatis Strains and Effects of Lemon and Ginger Essential Oils on Biofilm Formation, Indian Journal of Pharmaceutical Education and Research, (2016), 50 (2), 82-88.
• Avcioglu, N.H., Seyis Bilkay, I., Resistance, multidrug resistance and enterobacterial repetitive intergenic consensus polymerase chain reaction profiles of clinically important Klebsiella species, Asian Biomedicine, (2016), 10 (1), 41-47.
• Diken Gür S., Aksöz, N., “Molecular typing of clinical Pseudomonas aeruginosa strains by using RAPD-PCR”, Minerva Biotecnologica, (2016), 28(2); 104-13.
• Bilen Ozyurek, S., Seyis Bilkay I., Determining the distribution of the bla (VIM) gene in imipenem-resistant Pseudomonas aeruginosa strains isolated from three different hospitals, Minerva Biotecnologica, (2016), 28, 52-58.
• Bilen Ozyurek, S., Diken Gur, S., Seyis Bilkay, I. Investigation of antimicrobial activity of pyocyanin produced by Pseudomonas aeruginosa strains isolated from different clinical specimens, Hacettepe Journal of Biology & Chemistry, (2016), 44 (1) , 1-6.
• Bilen Ozyurek, S., Seyis Bilkay I. Determination of petroleum biodegradation by bacteria isolated from drilling fluid, waste mud pit and crude oil. Turkish Journal of Biochemistry, (2017), 42 (6), 609–616. https://doi.org/10.1515/tjb-2017-0087.
• Bilen Ozyurek, S., Seyis Bilkay, I., Determination of petroleum biodegradation by oil field isolated microorganisms, Journal of Biotechnology, (2017), 256, S23. (Abstract)
• Diken Gur, S., Bilen Ozyurek, S., Seyis Bilkay, I. Antimicrobial activity of thyme and rosemary oils against Pseudomonas aeruginosa strains, Hacettepe Journal of Biology & Chemistry, (2017), 45 (3), 2017, 435– 442.
• Avcioglu, N.H., Seyis Bilkay, I., Removal of Cyanide Compounds and Soil Cyanide by Klebsiella oxytoca, Hacettepe J. Biol. & Chem., (2017), 45 (4), 523–531.
• Bilen Ozyurek, S., Seyis Bilkay, I. Biodegradation of petroleum by Klebsiella pneumoniae isolated from drilling fluid, International Journal of Environmental Science and Technology, (2018), 15, 2107-2116. https://doi.org/10.1007/s13762-017-1581-y.
• Diken Gur S., Idil N., Aksöz N., ''Optimization of Enzyme Co-immobilization with Sodium Alginate and Glutaraldehyde-Activated Chitosan Beads'', Applied Biochemistry and Biotechnology, (2018), 184 (2); 538-552.
• Sahal G., Bilkay Seyis I.; Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm forming Candida isolates, Journal of the Brazilian Society of Tropical Medicine, (2018), 51(5), 644-650.
• Diken Gür S., Bakhshpour M., Denizli A., “Selective Detection of Escherichia coli Caused UTIs with Surface Imprinted Plasmonic Nanoscale Sensor”, Materials Science and Engineering:C, (2019), 104; 109869.
• Sahal, G., Nasseri, B., Ebrahimi, A., Bilkay, I.S.; Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates. Biotechnol Lett, (2019), 41(4-5): 511-522.
• Avcioglu, NH, Seyis Bilkay, I., Removal in Electroplating, Metal Plating and Gold Mining Industries' Wastewaters by Using Klebsiella pneumoniae and Klebsiella oxytoca Species, European Journal of Biology, (2019), 78(1): 5-10, .
• Bilen Ozyurek, S., Avcioglu, N.H., The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes, European Journal of Biology, 2020 (In Press).
• Bilen Ozyurek, S., Seyis Bilkay, I. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum‑contaminated waste mud pit, SN Applied Sciences, (2020), 2(272), 1-12. https://doi.org/10.1007/s42452-020-2044-5.
• Sahal, G., Woerdenbag, H. J., Hinrichs, W. L. J., Visser, A., Tepper, P. G., Quax, W. J., van der Mei, H.C., Bilkay, I.S.; Antifungal and biofilm inhibitory effect of Cymbopogon citratus (lemongrass) essential oil on biofilm forming by Candida tropicalis isolates; an in vitro study. J Ethnopharmacol, (2020), 246: 112188.
• Donmez H.G., Sahal, G., Akgor, U., Cagan, M., Ozgul, N., Beksac, M.S.; The relationship between the presence of HPV infection and biofilm formation in cervicovaginal smears. Infection (2020), 48(5), 735-740.
• Sahal, G., Woerdenbag, H. J., Hinrichs, W. L. J., Visser, A., van der Mei, H. C. and Bilkay, I.S.; Candida Biofilm Formation Assay on Essential Oil Coated Silicone Rubber. Bio-Protocol (In Press).

Links

About

Research Infrastructure

• Hot Water Bath (Memert Brand)
• Microscope (2 Pieces) (Leica Brand)
• Centrifuge (Eppendorf Brand 5417c Model)
• Refrigerator (AEG Brand)
• Vortex (Ika Brand Genius 3 Mode)
• Precision Balance (Ika Brand Rct Basic Model)
• Precision Balance (Yellowline Brand Mst Digital Model)
• pH Meter (Schott Brand)
• pH Meter (Consort Brand)
• Magnetic Stirrer with Heating

Researchers

•    Prof. Dr. Işıl Seyis Bilkay
•    Araş. Gör. Dr. Hande Avcıoğlu
•    Araş. Gör. Dr. Sinem Diken Gür
•    Araş. Gör. Dr. Sezen Bilen Özyürek
•    Araş. Gör. Dr. Gülcan Şahal
•    Meriç Birben
•    Cansu Sevim
•    Cansu Köten

Ongoing Projects

• Evaluation of Mycoremediation Potentials of Fungi in terms of Petroleum Biodegradation Biosorption and Biosurfactant Production, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2019-Continuing.)
• Determination of Glycoprotein with Surface Plasmon Nanosensor, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2019-Continuing.)
• Investigation of Antimicrobial and Antibiofilm Effects of Biosurfactants to be Produced with Industrial Wastes, TÜBİTAK 2209/A UNIVERSITY STUDENTS RESEARCH PROJECTS SUPPORT PROGRAM, 1919B012000647, (Continued).
• Investigation of antimicrobial and antibiofilm effects and cytotoxic potentials of hydrogels and microbial celluloses containing herbal substances, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, (2020-Continuing).

Completed Projects

• Investigation of Cyanide Biodegradation by Klebsiella Pneumoniae and Klebsiella Ornithinolytica Species, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2015.
• Coimmobilized Enzyme Design with Two Different Support Materials, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2015.
• Coimmobilized Enzyme Design for Wastewater Pretreatment, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Thesis Project (PhD), 2016.
• Investigation of petroleum biodegradation by bacteria isolated from crude oil, drilling mud and mud pit, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2016.
• Investigation of Biofilms Formed by Different Clinical Candida Strains on Various Materials and Different Herbal Substances that Have an Effect on These Biofilms, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, PhD Thesis Project, 2016.
• Investigation of Cyanide Biodegradation by Fungal Sources, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2017.
• Investigation of the biodegradation of microorganisms isolated from crude oil, drilling mud and mud pit and various hydrocarbons, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, PhD Thesis Project, 2017.
• Investigation of the effects of bacterial consortia adapted to oiled sludge pit on petroleum biodegradation, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, (2017-2018).
• Escherichia coli Determination by Surface Plasmon Resonance Biosensor, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2018.
• Investigation of Antibiofilm Effects of Polycaprolactone Nanofibers Containing Various Essential Oils on Different Candida Species, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, Rapid Support Project, 2018.
• Investigation of Biosurfactant Production by Bacterial Strains to be Isolated from Oil Field, TÜBİTAK 2209/A UNIVERSITY STUDENTS RESEARCH PROJECTS SUPPORT PROGRAM, 1919B011900736, (2019-2020).
• Research on the Use of Cellulose in Cyanide and Dye Removal, HACETTEPE UNIVERSITY SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT, 2020.

Publications

• Avcioglu, N.H., Sahal, G., Bilkay, I., Investigation of Biofilm Formation in Clinically Acquired Escherichia coli Strains, Hacettepe J. Biol. & Chem., (2015), 43 (1), 43–48,
• Avcioglu, N.H., Seyis Bilkay, I., Assessment of five clinical Klebsiella isolates in terms of antibiotic resistance and plasmid profiles, Turkish Journal of Biochemistry, (2015), 40 (6), 448-455,
• Bilen Ozyurek, S., Seyis Bilkay, I. Determination of resistance in Pseudomonas aeruginosa strains against beta lactam aminoglycoside and ciprofloxacin group antibiotics, Hacettepe Journal of Biology & Chemistry, (2015), 43, 59-67.
• Sahal G., Nasseri B., Bilkay Seyis I., Piskin E. “Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces Against Clinical P. mirabilis, C. glabrata and C. tropicalis Strains. ” J Appl Biomater Funct Mater., (2015), 13(4), 351-355.
• Sahal G., Bilkay Seyis I.; Multidrug resistance by biofilm-forming clinical strains of Proteus mirabilis., Asian Biomedicine (2015), 9(4), 535-541.
• Avcioglu, NH, Sahal, G., Bilkay, I., Antibiofilm Effects of Citrus limonum and Zingiber officinale oils on biofilm formation of Klebsiella ornithinolytica, Klebsiella oxytoca and Klebsiella terrigena species, African Journal of Traditional, Complementary and Alternative Medicines, (2016) ), 13(6):61-67.
• Avcioglu, N.H., Seyis Bilkay, I., Biological Treatment of Cyanide by Using Klebsiella pneumoniae Species, Food Technology and Biotechnology, (2016), 54 (4), 450-454.
• Sahal, G., Avcioglu, NH, Seyis Bilkay, I., Higher Biofilm Formation by Multi-Drug Resistant K. pneumoniae and K. rhinoscleromatis Strains and Effects of Lemon and Ginger Essential Oils on Biofilm Formation, Indian Journal of Pharmaceutical Education and Research, (2016), 50 (2), 82-88.
• Avcioglu, N.H., Seyis Bilkay, I., Resistance, multidrug resistance and enterobacterial repetitive intergenic consensus polymerase chain reaction profiles of clinically important Klebsiella species, Asian Biomedicine, (2016), 10 (1), 41-47.
• Diken Gür S., Aksöz, N., “Molecular typing of clinical Pseudomonas aeruginosa strains by using RAPD-PCR”, Minerva Biotecnologica, (2016), 28(2); 104-13.
• Bilen Ozyurek, S., Seyis Bilkay I., Determining the distribution of the bla (VIM) gene in imipenem-resistant Pseudomonas aeruginosa strains isolated from three different hospitals, Minerva Biotecnologica, (2016), 28, 52-58.
• Bilen Ozyurek, S., Diken Gur, S., Seyis Bilkay, I. Investigation of antimicrobial activity of pyocyanin produced by Pseudomonas aeruginosa strains isolated from different clinical specimens, Hacettepe Journal of Biology & Chemistry, (2016), 44 (1) , 1-6.
• Bilen Ozyurek, S., Seyis Bilkay I. Determination of petroleum biodegradation by bacteria isolated from drilling fluid, waste mud pit and crude oil. Turkish Journal of Biochemistry, (2017), 42 (6), 609–616. https://doi.org/10.1515/tjb-2017-0087.
• Bilen Ozyurek, S., Seyis Bilkay, I., Determination of petroleum biodegradation by oil field isolated microorganisms, Journal of Biotechnology, (2017), 256, S23. (Abstract)
• Diken Gur, S., Bilen Ozyurek, S., Seyis Bilkay, I. Antimicrobial activity of thyme and rosemary oils against Pseudomonas aeruginosa strains, Hacettepe Journal of Biology & Chemistry, (2017), 45 (3), 2017, 435– 442.
• Avcioglu, N.H., Seyis Bilkay, I., Removal of Cyanide Compounds and Soil Cyanide by Klebsiella oxytoca, Hacettepe J. Biol. & Chem., (2017), 45 (4), 523–531.
• Bilen Ozyurek, S., Seyis Bilkay, I. Biodegradation of petroleum by Klebsiella pneumoniae isolated from drilling fluid, International Journal of Environmental Science and Technology, (2018), 15, 2107-2116. https://doi.org/10.1007/s13762-017-1581-y.
• Diken Gur S., Idil N., Aksöz N., ''Optimization of Enzyme Co-immobilization with Sodium Alginate and Glutaraldehyde-Activated Chitosan Beads'', Applied Biochemistry and Biotechnology, (2018), 184 (2); 538-552.
• Sahal G., Bilkay Seyis I.; Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm forming Candida isolates, Journal of the Brazilian Society of Tropical Medicine, (2018), 51(5), 644-650.
• Diken Gür S., Bakhshpour M., Denizli A., “Selective Detection of Escherichia coli Caused UTIs with Surface Imprinted Plasmonic Nanoscale Sensor”, Materials Science and Engineering:C, (2019), 104; 109869.
• Sahal, G., Nasseri, B., Ebrahimi, A., Bilkay, I.S.; Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates. Biotechnol Lett, (2019), 41(4-5): 511-522.
• Avcioglu, NH, Seyis Bilkay, I., Removal in Electroplating, Metal Plating and Gold Mining Industries' Wastewaters by Using Klebsiella pneumoniae and Klebsiella oxytoca Species, European Journal of Biology, (2019), 78(1): 5-10, .
• Bilen Ozyurek, S., Avcioglu, N.H., The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes, European Journal of Biology, 2020 (In Press).
• Bilen Ozyurek, S., Seyis Bilkay, I. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum‑contaminated waste mud pit, SN Applied Sciences, (2020), 2(272), 1-12. https://doi.org/10.1007/s42452-020-2044-5.
• Sahal, G., Woerdenbag, H. J., Hinrichs, W. L. J., Visser, A., Tepper, P. G., Quax, W. J., van der Mei, H.C., Bilkay, I.S.; Antifungal and biofilm inhibitory effect of Cymbopogon citratus (lemongrass) essential oil on biofilm forming by Candida tropicalis isolates; an in vitro study. J Ethnopharmacol, (2020), 246: 112188.
• Donmez H.G., Sahal, G., Akgor, U., Cagan, M., Ozgul, N., Beksac, M.S.; The relationship between the presence of HPV infection and biofilm formation in cervicovaginal smears. Infection (2020), 48(5), 735-740.
• Sahal, G., Woerdenbag, H. J., Hinrichs, W. L. J., Visser, A., van der Mei, H. C. and Bilkay, I.S.; Candida Biofilm Formation Assay on Essential Oil Coated Silicone Rubber. Bio-Protocol (In Press).

Links