Medical Microbiology Department Bacteriology I Laboratory

About

Research Infrastructure

• -20℃ refrigerator (2 units)
• Refrigerator (2 units)
• Oven, Densitometry
• Vortex
• Automatic pipettes

Researchers

•    Prof. Dr. Özgen Eser (Laboratuvar Sorumlusu)
•    Öznur Gürpınar (Doktora öğrencisi)
•    Mukadder Güner (Teknisyen)

Ongoing Projects

• Analysis of Pseudomonas aeruginosa Isolates in Cystic Fibrosis Patients: Antibiotic susceptibility, resistance genes, clonal analysis and antibiofilm activity Project Coordinator: Özgen Eser, Researchers involved in the project: Öznur Gürpınar, Ishtar Dolapçı, Alper Tekeli, Didem Kart, Deniz Doğru Ersöz, Ebru Yalçın, Ugur Ozcelik, Nagehan Emiralioglu, Beste Ozsezen
• Antibiotic resistance, virulence and biofilm properties in Stenotrophomonas maltophilia, Project Coordinator: Özgen Eser, Project researchers: Öznur Gürpınar, Aybüke Özyar, Zeynep Ceren Karahan, Sedat Vezir

Completed Projects

• Investigation of Antibacterial Efficacy of Boron Nitride Coated Implants in an Osteomyelitis Model (Fast Support Project)
• Researchers in the Project: Ömür Çağlar (Executive), Gaye Özgür Çakal, Bilgin Kaftanoğlu, Özgen Eser, Samet Işık, Mustafa Fevzi Sargon, Project Duration: 2018-2020
• Global Pneumococcal Sequencing Project (International Project), Researchers in the Project: Stephen Bentley (Executive), Lesley McGee (Executive), Özgen Eser,…, Project Duration: 2014-2020
• In vitro determination of antibiotic susceptibility of Klebsiella spp, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa isolates isolated from urine cultures of hospitalized patients and investigation of mechanisms of resistance to fosfomycin (Quick Support Project), Project Researchers: Gülşen Readyolan (Executive), Deniz Gür, Şeyma Nigiz , Özgen Eser, Project Duration: 2018- 2019
• Evaluation of periodontal status and clinical immunological microbiological data in patients with cystic fibrosis (Quick Support Project), Researchers in the Project: Emil Tuğba Ataman Duruel (Executive), Fevzi İlhan Tezcan, Çağman Tan, Özgen Eser, Sanem Eşref, Onurcem Duruel, Project Duration 2018-2019
• The Relationship of ST131 H30 Clone Subclone with Virulence in Quinolone Resistant and Susceptible Invasive Escherichia coli Blood Isolates (Tübitak Project), Researchers in the Project: Özgen Eser (Executive), Öznur Gürpınar, Pınar Zarakolu, Gökhan Metan, Project Duration: 2017-2018
• Rapid Diagnosis of Carbapenemase Presence in Invasive Escherichia coli Blood Isolates by Immunochromatographic Method (Quick Support Project), Project Researchers: Özgen Eser (Coordinator), Pınar Zarakolu Köşker, Öznur Gürpınar, Project Duration: 2017-2018
• Tigecycline susceptibility and presence of biofilm in multidrug resistant Acinetobacter baumannii isolates (Fast Support Project), Project Researchers: Alper Ergin (Executive), Özge Altınok, Özgen Eser, Project Duration: 2015-2017
• Surveillance of quinolone-resistant Escherichia coli in neutropenic patients with hematological cancer receiving levofloxacin prophylaxis (Fast Support Project), Project Researchers: Murat Akova (Coordinator), Sezgin Etgül, Pınar Zarakolu, Özgen Eser, Project Duration: 2015-2016

Publications

•    Demirci-Duarte S., Unalan-Altintop T.,Koseoglu E.,Cakar A.,Altun B.,Sancak B.,Gur D., Prevalence of O25b-ST131 clone and fosfomycin resistance in urinary Escherichia coli isolates and their relation to CTX-M determinant., Diagnostic microbiology and infectious disease, cilt.98, ss.115098,2020
•    Gladstone RA, Lo SW, Goater R, Yeats C, Taylor B, Hadfield J, Lees JA, Croucher NJ, van Tonder AJ, Bentley LJ, Quah FX, Blaschke AJ, Pershing NL, Byington CL, Balaji V, Hryniewicz W, Sigauque B, Ravikumar KL, Almeida SCG, Ochoa TJ, Ho PL, du Plessis M, Ndlangisa KM, Cornick JE, Kwambana-Adams B, Benisty R, Nzenze SA, Madhi SA, Hawkins PA, Pollard AJ, Everett DB, Antonio M, Dagan R, Klugman KP, von Gottberg A, Metcalf BJ, Li Y, Beall BW, McGee L, Breiman RF, Aanensen DM, Bentley SD; The Global Pneumococcal Sequencing Consortium., Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates., Microb Genom. 2020 May;6(5):e000357
•    Duruel O., Berker E., Özşin-Özler C., Gharibzadeh-Hızal M., Gürpınar Ö., Eryılmaz-Polat S., Ataman-Duruel E., Tan Ç.,Karabulut E., Tekçiçek M.,et al., Levels of pro- and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis., Cytokine, cilt.127, ss.154987,2020
•    Altınok Ö., Boral B., Ergin A., Eser Ö., Existence of Biofilm and Biofilm-Associated Virulence Genes inMulti-Drug Resistant Invasive Acinetobacter baumannii isolates., Mikrobiyoloji Bulteni, cilt.54, ss.40-49,2020
•    LoS., GladstoneR., VanT.,DuP., CornickJ., HawkinsP., MadhiS., NzenzeS., KandasamyR., RavikumarK.,etal., A mosaic tetracycline resistance gene tet(S/M) detected in an MDR pneumococcal CC230 lineage that underwent capsular switching in South Africa., Journal of Antimicrobial Chemotherapy, 2019
•    Aydogan T., Gurpinar Ö., Eser Ö., Mathyk B., Ergen A., A new look at the etiology of interstitial cystitis/bladder pain syndrome: extraordinary cultivations, International Urology and Nephrology, cilt.51, ss.1961-1967,2019
•    Boral B., Unaldi Ö., Ergin A., Durmaz R., Eser Ö., A prospective multicenter study on the evaluation of antimicrobial resistance and molecular epidemiology ofmultidrug-resistant Acinetobacter baumanni infections in intensive care units with clinical and environmental features., Annals of Clinical Microbiology and Antimicrobials, cilt.18, ss.19,2019
•    Lo S., Gladstone R., Van T., Lees J., Du P., Benisty R., Givon-Lavi N., Hawkins P.,Cornick J., Kwambana-Adams B.,et al., Pneumococcal lineages associated with serotype replacement and antibiotic resistance in childhood invasive pneumococcal disease in the post-PCV13 era: an internationalwhole-genome sequencing study., The Lancet Infectious Diseases, cilt.19, ss.759-769,2019
•    Van T., Gladstone R.,Lo S.,Nahm M.,Du P.,Cornick J.,KwambanaAdams B.,Madhi S.,Hawkins P.,Benisty R.,et al., Putative novel cps loci in a large global col ection of pneumococci., Microbial genomics, cilt.5,2019
•    Gladstone R., Lo S., Lees J.,Croucher N., Van T., Corander J., Page A., Martinen P., Bentley L., Ochoa T., et al., International genomic definition of pneumococcal lineages, to contextualise disease, antibiotic resistance and vaccine impact., EBio Medicine, cilt.43, ss.338-346,2019
•    Epping L., Van T., Gladstone R., The G., Bentley S., Page A., Keane J., SeroBA: rapid high-throughput serotyping of Streptococcus pneumoniae fromwhole genome sequence data., Microbial genomics, cilt.4,2018 12.
•    LoS., GladstoneR., VanT., Hawkins P., KwambanaAdams B.,CornickJ., MadhiS., NzenzeS., DuP., Kandasamy R., et al., Global Distribution of Invasive Serotype 35D Streptococcus pneumoniae Isolates following Introduction of 13-Valent Pneumococcal Conjugate Vaccine., Journal Clinical Microbiology, cilt.56,2018
•    Suzuk Yıldız S., Simsek H., Coplu N, Gulay Z, Uamdss C.G., National Antimicrobial Resistance Surveilance System (NAMRSS) external quality assessment studies: 2011-2016., Mikrobiyoloji Bulteni, cilt.51, ss.247-259,2017
•    Metan G., Ilbay A., Eser O.K., Ünal S., Zarakolu P., A Silent Epidemic of Colistin- and Carbapenem-Resistant Enterobacteriaceae at a Turkish University Hospital Infection Control and Hospital Epidemiology, cilt.38, ss.254-257,2017
•    Zarakolu P., Eser O.K., Aladag E., Al-Zahranı I.A., Day K.M., Atmaca O., Boral B., Çakır B., Perry J.D., Akova M., Epidemiology of carbapenem-resistant Klebsiel a pneumoniae colonization: a surveil ance study at a Turkish university hospital from2009 to 2013, Diagnositc Microbiology and Infectious Disease, cilt.85, ss.466-470,2016
•    Bakteriyel Biyofilmler. Özge Altınok, Öznur Gürpınar, Özgen Eser. Özge ALTINOK, Öznur GÜRPINAR, Özgen ESER. Tıp Fakültesi Klinikleri Dergisi, 2018; Cilt1, Sayı 2, 45 – 51.
•    Kistik fibrozis periodontal hastalık ilişkisinin klinik ve mikrobiyolojik olarak değerlendirilmesi. Onurcem Duruel, Ezel Berker, Cansu Özşin Özler, Mina Gharibzadeh-Hızal, Öznur Gürpınar, Sanem Eryılmaz-Polat, Emel Tuğba Ataman-Duruel, Meryem Tekçiçek, Özgen Eser, Nural Kiper. Klimud 2019 Kongresi. Poster Bildirisi.
•    İnvaziv ve noninvaziv Acinetobacter baumannii klinik izolatlarında biyofilm yapımı ve virülans ilişkisi. Öznur Gürpınar, Alper Ergin, Pınar Zarakolu, Özgen Eser. TMC 2018 Kongresi. Sözlü Bildiri.
•    Peynir Üretim Aşamalarından Elde Edilmiş Enterobacteriaceae İzolatlarında Antibiyotik Direnç Genlerinin Varlığı ve Isısal Direnç. Seda Özdikmenli Tepeli, Özgen Eser, Öznur Gürpınar, Yeşim Beşli, Nükhet Nilüfer Zorba. TMC 2018 Kongresi. Sözlü Bildiri.
•    İnterstisyel Sistit Etiyolojisinde Kapsamlı Mikrobiyolojik Tanının Rolü: İleriye Dönük Bir Vaka-Kontrol Çalışması. Tahsin Batuhan Aydoğan, Öznur Gürpınar, Özgen Eser, Ali Ergen. Ankem 2018 Kongresi. Sözlü Bildiri.
•    Kinolon dirençli ve duyarlı invaziv Escherichia coli kan izolatlarında ST131/H30 Klon/Subklonunun virulans ile ilişkisi. Öznur Gürpınar, Ahmet İlbay, Gökhan Metan, Pınar Zarakolu, Özgen Köseoğlu Eser. Klimud 2017 Kongresi. Sözlü Bildiri.
•    İnvaziv Escherichia coli kan izolatlarında karbapenemaz varlığının hızlı tanısı. Öznur Gürpınar, Pınar Zarakolu, Özgen Köseoğlu Eser. Poster Bildirisi.
•    Kan yolu enfeksiyonlarından izole edilen Escherichia coli’lerde karbapenem direnç genleri ve ST131/H30 varlığı. Öznur Gürpınar, Ahmet İlbay, Gökhan Metan, Pınar Zarakolu, Özgen Köseoğlu Eser. TMC 2016 Kongresi. Sözlü Bildiri.
•    Çok ilaca dirençli Acinetobacter baumannii izolatlarında tigesiklin duyarlılığı. TMC 2016 Kongresi. Poster Bildirisi. Öznur Gürpınar, Barış Boral, Alper Ergin, Özgen Köseoğlu Eser.

Links

About

Research Infrastructure

• -20℃ refrigerator (2 units)
• Refrigerator (2 units)
• Oven, Densitometry
• Vortex
• Automatic pipettes

Researchers

•    Prof. Dr. Özgen Eser (Laboratuvar Sorumlusu)
•    Öznur Gürpınar (Doktora öğrencisi)
•    Mukadder Güner (Teknisyen)

Ongoing Projects

• Analysis of Pseudomonas aeruginosa Isolates in Cystic Fibrosis Patients: Antibiotic susceptibility, resistance genes, clonal analysis and antibiofilm activity Project Coordinator: Özgen Eser, Researchers involved in the project: Öznur Gürpınar, Ishtar Dolapçı, Alper Tekeli, Didem Kart, Deniz Doğru Ersöz, Ebru Yalçın, Ugur Ozcelik, Nagehan Emiralioglu, Beste Ozsezen
• Antibiotic resistance, virulence and biofilm properties in Stenotrophomonas maltophilia, Project Coordinator: Özgen Eser, Project researchers: Öznur Gürpınar, Aybüke Özyar, Zeynep Ceren Karahan, Sedat Vezir

Completed Projects

• Investigation of Antibacterial Efficacy of Boron Nitride Coated Implants in an Osteomyelitis Model (Fast Support Project)
• Researchers in the Project: Ömür Çağlar (Executive), Gaye Özgür Çakal, Bilgin Kaftanoğlu, Özgen Eser, Samet Işık, Mustafa Fevzi Sargon, Project Duration: 2018-2020
• Global Pneumococcal Sequencing Project (International Project), Researchers in the Project: Stephen Bentley (Executive), Lesley McGee (Executive), Özgen Eser,…, Project Duration: 2014-2020
• In vitro determination of antibiotic susceptibility of Klebsiella spp, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa isolates isolated from urine cultures of hospitalized patients and investigation of mechanisms of resistance to fosfomycin (Quick Support Project), Project Researchers: Gülşen Readyolan (Executive), Deniz Gür, Şeyma Nigiz , Özgen Eser, Project Duration: 2018- 2019
• Evaluation of periodontal status and clinical immunological microbiological data in patients with cystic fibrosis (Quick Support Project), Researchers in the Project: Emil Tuğba Ataman Duruel (Executive), Fevzi İlhan Tezcan, Çağman Tan, Özgen Eser, Sanem Eşref, Onurcem Duruel, Project Duration 2018-2019
• The Relationship of ST131 H30 Clone Subclone with Virulence in Quinolone Resistant and Susceptible Invasive Escherichia coli Blood Isolates (Tübitak Project), Researchers in the Project: Özgen Eser (Executive), Öznur Gürpınar, Pınar Zarakolu, Gökhan Metan, Project Duration: 2017-2018
• Rapid Diagnosis of Carbapenemase Presence in Invasive Escherichia coli Blood Isolates by Immunochromatographic Method (Quick Support Project), Project Researchers: Özgen Eser (Coordinator), Pınar Zarakolu Köşker, Öznur Gürpınar, Project Duration: 2017-2018
• Tigecycline susceptibility and presence of biofilm in multidrug resistant Acinetobacter baumannii isolates (Fast Support Project), Project Researchers: Alper Ergin (Executive), Özge Altınok, Özgen Eser, Project Duration: 2015-2017
• Surveillance of quinolone-resistant Escherichia coli in neutropenic patients with hematological cancer receiving levofloxacin prophylaxis (Fast Support Project), Project Researchers: Murat Akova (Coordinator), Sezgin Etgül, Pınar Zarakolu, Özgen Eser, Project Duration: 2015-2016

Publications

•    Demirci-Duarte S., Unalan-Altintop T.,Koseoglu E.,Cakar A.,Altun B.,Sancak B.,Gur D., Prevalence of O25b-ST131 clone and fosfomycin resistance in urinary Escherichia coli isolates and their relation to CTX-M determinant., Diagnostic microbiology and infectious disease, cilt.98, ss.115098,2020
•    Gladstone RA, Lo SW, Goater R, Yeats C, Taylor B, Hadfield J, Lees JA, Croucher NJ, van Tonder AJ, Bentley LJ, Quah FX, Blaschke AJ, Pershing NL, Byington CL, Balaji V, Hryniewicz W, Sigauque B, Ravikumar KL, Almeida SCG, Ochoa TJ, Ho PL, du Plessis M, Ndlangisa KM, Cornick JE, Kwambana-Adams B, Benisty R, Nzenze SA, Madhi SA, Hawkins PA, Pollard AJ, Everett DB, Antonio M, Dagan R, Klugman KP, von Gottberg A, Metcalf BJ, Li Y, Beall BW, McGee L, Breiman RF, Aanensen DM, Bentley SD; The Global Pneumococcal Sequencing Consortium., Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates., Microb Genom. 2020 May;6(5):e000357
•    Duruel O., Berker E., Özşin-Özler C., Gharibzadeh-Hızal M., Gürpınar Ö., Eryılmaz-Polat S., Ataman-Duruel E., Tan Ç.,Karabulut E., Tekçiçek M.,et al., Levels of pro- and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis., Cytokine, cilt.127, ss.154987,2020
•    Altınok Ö., Boral B., Ergin A., Eser Ö., Existence of Biofilm and Biofilm-Associated Virulence Genes inMulti-Drug Resistant Invasive Acinetobacter baumannii isolates., Mikrobiyoloji Bulteni, cilt.54, ss.40-49,2020
•    LoS., GladstoneR., VanT.,DuP., CornickJ., HawkinsP., MadhiS., NzenzeS., KandasamyR., RavikumarK.,etal., A mosaic tetracycline resistance gene tet(S/M) detected in an MDR pneumococcal CC230 lineage that underwent capsular switching in South Africa., Journal of Antimicrobial Chemotherapy, 2019
•    Aydogan T., Gurpinar Ö., Eser Ö., Mathyk B., Ergen A., A new look at the etiology of interstitial cystitis/bladder pain syndrome: extraordinary cultivations, International Urology and Nephrology, cilt.51, ss.1961-1967,2019
•    Boral B., Unaldi Ö., Ergin A., Durmaz R., Eser Ö., A prospective multicenter study on the evaluation of antimicrobial resistance and molecular epidemiology ofmultidrug-resistant Acinetobacter baumanni infections in intensive care units with clinical and environmental features., Annals of Clinical Microbiology and Antimicrobials, cilt.18, ss.19,2019
•    Lo S., Gladstone R., Van T., Lees J., Du P., Benisty R., Givon-Lavi N., Hawkins P.,Cornick J., Kwambana-Adams B.,et al., Pneumococcal lineages associated with serotype replacement and antibiotic resistance in childhood invasive pneumococcal disease in the post-PCV13 era: an internationalwhole-genome sequencing study., The Lancet Infectious Diseases, cilt.19, ss.759-769,2019
•    Van T., Gladstone R.,Lo S.,Nahm M.,Du P.,Cornick J.,KwambanaAdams B.,Madhi S.,Hawkins P.,Benisty R.,et al., Putative novel cps loci in a large global col ection of pneumococci., Microbial genomics, cilt.5,2019
•    Gladstone R., Lo S., Lees J.,Croucher N., Van T., Corander J., Page A., Martinen P., Bentley L., Ochoa T., et al., International genomic definition of pneumococcal lineages, to contextualise disease, antibiotic resistance and vaccine impact., EBio Medicine, cilt.43, ss.338-346,2019
•    Epping L., Van T., Gladstone R., The G., Bentley S., Page A., Keane J., SeroBA: rapid high-throughput serotyping of Streptococcus pneumoniae fromwhole genome sequence data., Microbial genomics, cilt.4,2018 12.
•    LoS., GladstoneR., VanT., Hawkins P., KwambanaAdams B.,CornickJ., MadhiS., NzenzeS., DuP., Kandasamy R., et al., Global Distribution of Invasive Serotype 35D Streptococcus pneumoniae Isolates following Introduction of 13-Valent Pneumococcal Conjugate Vaccine., Journal Clinical Microbiology, cilt.56,2018
•    Suzuk Yıldız S., Simsek H., Coplu N, Gulay Z, Uamdss C.G., National Antimicrobial Resistance Surveilance System (NAMRSS) external quality assessment studies: 2011-2016., Mikrobiyoloji Bulteni, cilt.51, ss.247-259,2017
•    Metan G., Ilbay A., Eser O.K., Ünal S., Zarakolu P., A Silent Epidemic of Colistin- and Carbapenem-Resistant Enterobacteriaceae at a Turkish University Hospital Infection Control and Hospital Epidemiology, cilt.38, ss.254-257,2017
•    Zarakolu P., Eser O.K., Aladag E., Al-Zahranı I.A., Day K.M., Atmaca O., Boral B., Çakır B., Perry J.D., Akova M., Epidemiology of carbapenem-resistant Klebsiel a pneumoniae colonization: a surveil ance study at a Turkish university hospital from2009 to 2013, Diagnositc Microbiology and Infectious Disease, cilt.85, ss.466-470,2016
•    Bakteriyel Biyofilmler. Özge Altınok, Öznur Gürpınar, Özgen Eser. Özge ALTINOK, Öznur GÜRPINAR, Özgen ESER. Tıp Fakültesi Klinikleri Dergisi, 2018; Cilt1, Sayı 2, 45 – 51.
•    Kistik fibrozis periodontal hastalık ilişkisinin klinik ve mikrobiyolojik olarak değerlendirilmesi. Onurcem Duruel, Ezel Berker, Cansu Özşin Özler, Mina Gharibzadeh-Hızal, Öznur Gürpınar, Sanem Eryılmaz-Polat, Emel Tuğba Ataman-Duruel, Meryem Tekçiçek, Özgen Eser, Nural Kiper. Klimud 2019 Kongresi. Poster Bildirisi.
•    İnvaziv ve noninvaziv Acinetobacter baumannii klinik izolatlarında biyofilm yapımı ve virülans ilişkisi. Öznur Gürpınar, Alper Ergin, Pınar Zarakolu, Özgen Eser. TMC 2018 Kongresi. Sözlü Bildiri.
•    Peynir Üretim Aşamalarından Elde Edilmiş Enterobacteriaceae İzolatlarında Antibiyotik Direnç Genlerinin Varlığı ve Isısal Direnç. Seda Özdikmenli Tepeli, Özgen Eser, Öznur Gürpınar, Yeşim Beşli, Nükhet Nilüfer Zorba. TMC 2018 Kongresi. Sözlü Bildiri.
•    İnterstisyel Sistit Etiyolojisinde Kapsamlı Mikrobiyolojik Tanının Rolü: İleriye Dönük Bir Vaka-Kontrol Çalışması. Tahsin Batuhan Aydoğan, Öznur Gürpınar, Özgen Eser, Ali Ergen. Ankem 2018 Kongresi. Sözlü Bildiri.
•    Kinolon dirençli ve duyarlı invaziv Escherichia coli kan izolatlarında ST131/H30 Klon/Subklonunun virulans ile ilişkisi. Öznur Gürpınar, Ahmet İlbay, Gökhan Metan, Pınar Zarakolu, Özgen Köseoğlu Eser. Klimud 2017 Kongresi. Sözlü Bildiri.
•    İnvaziv Escherichia coli kan izolatlarında karbapenemaz varlığının hızlı tanısı. Öznur Gürpınar, Pınar Zarakolu, Özgen Köseoğlu Eser. Poster Bildirisi.
•    Kan yolu enfeksiyonlarından izole edilen Escherichia coli’lerde karbapenem direnç genleri ve ST131/H30 varlığı. Öznur Gürpınar, Ahmet İlbay, Gökhan Metan, Pınar Zarakolu, Özgen Köseoğlu Eser. TMC 2016 Kongresi. Sözlü Bildiri.
•    Çok ilaca dirençli Acinetobacter baumannii izolatlarında tigesiklin duyarlılığı. TMC 2016 Kongresi. Poster Bildirisi. Öznur Gürpınar, Barış Boral, Alper Ergin, Özgen Köseoğlu Eser.

Links