Medical Microbiology Department Molecular Microbiology Lab I / II

About

Research Infrastructure

• Lab1: Ice maker, microwave oven, power supply for electrophoresis (2), precision balance (2), thermocycler (3), microcentrifuge (2), shaker, gel display device, refrigerator, biosafety cabinet, -20◦C freezer (3), water bath, midiscentrifuge, hybridization incubator
• Lab2: Refrigerator (3), -20◦C freezer (2), centrifuge, microcentrifuge, plate centrifuge, heat block, magnetic stirrer, vortex, water bath, biosafety cabinet, oven (2)

Researchers

• Assoc. Dr. Alpaslan Alp

• Prof. Dr. Ozgen Eser

• Prof. Dr. Zeynep Saribas

• Faculty members of the department

• Department research assistants

• PhD students

• Graduate students

 

Ongoing Projects

• 1) Identification of Mycobacteria species by MALDITOF MS method Sponsored by: Hacettepe University Scientific Research Unit, Project no: TSA-2018-17321
• 2) Determination of drug susceptibility pattern of Mycobacteroides (Mycobacterium) abscessus subspecies abscessus isolates, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: TYL-2019-18134
• Comparison of systemic and local inflammatory response in videothoracoscopic vats and open surgery by measuring serum pleural fluid and bronchoalveolar lavage cytokine levels in patients who underwent pulmonary resection by applying single lung ventilation for non-small cell lung cancer, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-18221
• Evaluation of the effects of clinical phenotypes caused by Aspergillus on respiratory functions in cystic fibrosis, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2020-18554
• Comparison of serum endocan icam1 and vegf levels before and after resection in non-small cell lung cancer, Supported by: Hacettepe University Scientific Research Unit, Project no: TTU-2020-18824
• Screening and Genotyping of Chronic Hepatitis C Acute Hepatitis Hematopoietic Stem Cell Transplantation Kidney Transplant Hepatitis E Virus in High Risk Patient Groups. Supporting organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-17551.
• Rapid detection of Staphylococcus aureus isolates from positive blood cultures. Supporting organization: Hacettepe University Scientific Research Unit, Project no: THD-2019-18350.
• Detection of MIC values ​​of new antituberculosis drugs bedaquiline and delamanid, and mutations causing resistance in clinical isolates of Mycobacterium tuberculosis. Supporting organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-18169.

Completed Projects

• 2015-2016 Detection of efflux pumps that cause isoniazid resistance in Mycobacterium tuberculosis complex clinical isolates, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD2015-7116
• Comparison of two different decontamination processes applied prior to 2017-2018 Mycobacterium tuberculosis isolation, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2017-12888
• Correlation of serum spd1 and spdl1 levels with clinical pathological features and lymph node metastasis in 2019-2020 early stage small cell female lung cancer patients, Supported by: Hacettepe University Scientific Research Unit, Project no: TTU-2019-18220
• The relationship between colon cancer and salivary microbiome, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2017-12288.
• Determining the role of viral agents in the etiology of cystic fibrosis patients with acute pulmonary exacerbation and investigating the effects of viruses on clinical laboratory and radiological parameters, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: THD-2017-15159.
• Can Good Clinical Course in COVID19 Positive Children Compared to Adults Be Explained by Cytokine and Chemokine Responses?, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2020-18774.

Publications

• Emiralioğu N, Doğru Ersöz D, Oğuz B, Sarıbaş Z, Yalçın E, Özçelik U, Özsürekçi Y, Cengiz AB, Kiper N. Pulmonary Mycobacterium abscessus infection in a patient with Triple A syndrome. Journal of Tropical Pediatrics 2016; 62:324-327 (SCI).
Prepared G, Sarıbaş Z, Arıkan-Akdağlı S. Comparison of Microdilution and Disk Diffusion Methods for Detection of Fluconazole and Voriconazole Susceptibility in Clinical Candida glabrata Isolates and Determination of the Change in Sensitivity Results with the New CLSI Resistance Limit Values. Bulletin of Microbiology 2016, 50:428-437 (SCI-EXP).
• Sarıbaş Z. Microbiological Diagnosis of Tuberculosis. Contribution Journal of Pediatrics 2018; 39 (3-4):329-334.
• Alp A, Sarıbaş Z. Evaluation of Anyplex MTB/NTM test in detecting Mycobacterium tuberculosis from clinical specimens. Bulletin of Microbiology 2019;53(4):355-363/doi: 10.5578/mb.67783 (SCI-EXP).
• Aykac K, Karadağ-Öncel E, Basaranoglu ST, Alp A, Cengiz AB, Ceyhan M, Kara A. Respiratory viral infections in infants with possible sepsis. J Med Virol 2019; 91:171-178. https://doi.org/10.1002/jmv.25309
• Güven DC, Dizdar Ö, Alp A, Akdoğan Kittana FN, Karakoç D, Hamaloğlu E, Laçin S, Karakaş Y, Kılıçkap S, Hayran M, Yalçın Ş. Analysis of Fusobacterium nucleatum and Streptococcus gallolyticus in saliva of colorectalcancer patients. Biomark Med 2019; 13 (9): 725-735. doi: 10.2217/bmm-2019-0020.

Links

About

Research Infrastructure

• Lab1: Ice maker, microwave oven, power supply for electrophoresis (2), precision balance (2), thermocycler (3), microcentrifuge (2), shaker, gel display device, refrigerator, biosafety cabinet, -20◦C freezer (3), water bath, midiscentrifuge, hybridization incubator
• Lab2: Refrigerator (3), -20◦C freezer (2), centrifuge, microcentrifuge, plate centrifuge, heat block, magnetic stirrer, vortex, water bath, biosafety cabinet, oven (2)

Researchers

• Assoc. Dr. Alpaslan Alp

• Prof. Dr. Ozgen Eser

• Prof. Dr. Zeynep Saribas

• Faculty members of the department

• Department research assistants

• PhD students

• Graduate students

 

Ongoing Projects

• 1) Identification of Mycobacteria species by MALDITOF MS method Sponsored by: Hacettepe University Scientific Research Unit, Project no: TSA-2018-17321
• 2) Determination of drug susceptibility pattern of Mycobacteroides (Mycobacterium) abscessus subspecies abscessus isolates, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: TYL-2019-18134
• Comparison of systemic and local inflammatory response in videothoracoscopic vats and open surgery by measuring serum pleural fluid and bronchoalveolar lavage cytokine levels in patients who underwent pulmonary resection by applying single lung ventilation for non-small cell lung cancer, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-18221
• Evaluation of the effects of clinical phenotypes caused by Aspergillus on respiratory functions in cystic fibrosis, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2020-18554
• Comparison of serum endocan icam1 and vegf levels before and after resection in non-small cell lung cancer, Supported by: Hacettepe University Scientific Research Unit, Project no: TTU-2020-18824
• Screening and Genotyping of Chronic Hepatitis C Acute Hepatitis Hematopoietic Stem Cell Transplantation Kidney Transplant Hepatitis E Virus in High Risk Patient Groups. Supporting organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-17551.
• Rapid detection of Staphylococcus aureus isolates from positive blood cultures. Supporting organization: Hacettepe University Scientific Research Unit, Project no: THD-2019-18350.
• Detection of MIC values ​​of new antituberculosis drugs bedaquiline and delamanid, and mutations causing resistance in clinical isolates of Mycobacterium tuberculosis. Supporting organization: Hacettepe University Scientific Research Unit, Project no: TSA-2019-18169.

Completed Projects

• 2015-2016 Detection of efflux pumps that cause isoniazid resistance in Mycobacterium tuberculosis complex clinical isolates, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD2015-7116
• Comparison of two different decontamination processes applied prior to 2017-2018 Mycobacterium tuberculosis isolation, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2017-12888
• Correlation of serum spd1 and spdl1 levels with clinical pathological features and lymph node metastasis in 2019-2020 early stage small cell female lung cancer patients, Supported by: Hacettepe University Scientific Research Unit, Project no: TTU-2019-18220
• The relationship between colon cancer and salivary microbiome, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2017-12288.
• Determining the role of viral agents in the etiology of cystic fibrosis patients with acute pulmonary exacerbation and investigating the effects of viruses on clinical laboratory and radiological parameters, Supporting Organization: Hacettepe University Scientific Research Unit, Project no: THD-2017-15159.
• Can Good Clinical Course in COVID19 Positive Children Compared to Adults Be Explained by Cytokine and Chemokine Responses?, Sponsored by: Hacettepe University Scientific Research Unit, Project no: THD-2020-18774.

Publications

• Emiralioğu N, Doğru Ersöz D, Oğuz B, Sarıbaş Z, Yalçın E, Özçelik U, Özsürekçi Y, Cengiz AB, Kiper N. Pulmonary Mycobacterium abscessus infection in a patient with Triple A syndrome. Journal of Tropical Pediatrics 2016; 62:324-327 (SCI).
Prepared G, Sarıbaş Z, Arıkan-Akdağlı S. Comparison of Microdilution and Disk Diffusion Methods for Detection of Fluconazole and Voriconazole Susceptibility in Clinical Candida glabrata Isolates and Determination of the Change in Sensitivity Results with the New CLSI Resistance Limit Values. Bulletin of Microbiology 2016, 50:428-437 (SCI-EXP).
• Sarıbaş Z. Microbiological Diagnosis of Tuberculosis. Contribution Journal of Pediatrics 2018; 39 (3-4):329-334.
• Alp A, Sarıbaş Z. Evaluation of Anyplex MTB/NTM test in detecting Mycobacterium tuberculosis from clinical specimens. Bulletin of Microbiology 2019;53(4):355-363/doi: 10.5578/mb.67783 (SCI-EXP).
• Aykac K, Karadağ-Öncel E, Basaranoglu ST, Alp A, Cengiz AB, Ceyhan M, Kara A. Respiratory viral infections in infants with possible sepsis. J Med Virol 2019; 91:171-178. https://doi.org/10.1002/jmv.25309
• Güven DC, Dizdar Ö, Alp A, Akdoğan Kittana FN, Karakoç D, Hamaloğlu E, Laçin S, Karakaş Y, Kılıçkap S, Hayran M, Yalçın Ş. Analysis of Fusobacterium nucleatum and Streptococcus gallolyticus in saliva of colorectalcancer patients. Biomark Med 2019; 13 (9): 725-735. doi: 10.2217/bmm-2019-0020.

Links