Muscle Physiology and Exercise Biochemistry Laboratory

About

Research Infrastructure

•   -80°C Freezer (Thermo scientific, Forma 8800 Series)
•   -20°C Deep Freeze (Thermo scientific, Forma)
•   Snow Machine (Scotsman AF 80)
•   Dry Heating Block (VLM HP2)
•   Homogenizer (Bullet Blender Storm, Next Advance)
•   Refrigerated Centrifuge (Hermle Z 323 K)
•   Microplate reader  (SpectraMax i3x, Molecular Devices)
•   Gel Electrophoresis System (BioRad 16x20 and miniblot system)
•   Semi-Dry Transfer System (Trans-Blot Turbo, BioRad)
•   Chemiluminescence Imaging device (BIO-RAD ChemiDoc TMMP Imaging System and Flour Chem E Protein Simple)
•   Real Time PCR (ViiATM 7 Real-Time PCR system, Applied Biosystems, Foster City, CA)
•   Thermal Cycling Device (Veriti®, Applied Biosystems, Foster City, CA)
•   Cryotome (Shandon Cryotome SME)

Researchers

  • Professor Haydar Demirel, M.D. Ph.D.
  • Associate Professor Senay Akin
  • Assistant Profesor Inci Tugce Çolluoglu (Karabuk University, Faculty of Medicine, Department of Cardiology,)
  • Doctor Levend Karaçoban, M.D., Ph.D. (Department of Sports Medicine, Faculty of Medicine)
  • Ümit Hayta (Ph. D. student)
  • Research Assistant İrem Güngör (Ph. D. student)
  • Doctor Yigitcan Menderes, M.D. (Department of Sports Medicine, Faculty of Medicine)
  • Research Assistant Kagan Mert Gulec (Master student)
  • Eda Aytekin (trainee, 2022, Bilkent University, Molecular Biology and Genetics Department)

Ongoing Projects

•   The Role of Tübitak 1001-Whole Body Vibration in Reversing Immobilization-Induced Skeletal Muscle Atrophy

•   Tübitak 1002- The Role of Exercise-Induced Hsp72 in Preventing Glucocorticoid-Induced Skeletal Muscle Atrophy

Completed Projects

• 014 D01 407 001 -501 (Support) The effect of long-term swimming exercise on heart muscle antioxidant defense mechanism and cytokines in young and old rats
•    TED-2017-15076 (Event Participation) The Effect of Whole Body Vibration and Immobilization on Protein Synthesis and Destruction Pathways
•   THD-2017-13130 (Fast Boost) Effect of Acute Long Term Nature Running on Plasma Myokine Levels
•   TYL-2016-8835 (Master's Degree) The Effect of Whole Body Vibration and Immobilization on Myosin Heavy Chain Profile and HSP72 Levels in Rat Skeletal Muscle

Publications

•   Akin S., Kubat GB., Guray U., Akin Y., Demirel HA. Possible Value of Galectin-3 on follow-up of cardiac remodeling during Glucocorticoid treatment. Journal of Biochemical and Molecular Toxicology. It has been accepted for publication.
•   Akin S., Kubat GB., Demirel HA. Exercise, mitochondrial biogenesis and disuse-induced atrophy. Turkish Journal of Sports Medicine. It has been accepted for publication.
•   Akin Ş., Ozerklig B., Demirel H.A. Exercise-Induced Rhabdomyolysis. Various Health Problems in Athletes. 1st Edition. Ankara: Turkey Clinics; 2020. p.13- 8.
•    Yargic MP., Torgutalp S., Akin S., Babayeva N., Torgutalp M., Demirel AH. Acute long-distance trail running increases serum IL-6, IL-15, and Hsp72 levels. Appl Physiol NutrMetab. 2018; 10.1139/apnm-2018-0520.
•    Akin Ş., Ozerklig B., Turkel I., Demirel H.A. Molecular Mechanisms Regulating Skeletal Muscle Mass. Turkish Journal of Sports Medicine, 10.5152/tjsm.2019.125.
•    Akin Ş., Donmez G., Ozdemir M., Demirel H.A. Does Whole Body Vibration Increase AMPK Activation Decreased by Immobilization? Turkish Journal of Sports Medicine 2018,10.5152/tjsm.2018.107.
•    Akin Ş., Demirel H.A. Sarcopenia and Exercise. Turkiye Klinikleri J Sports Med-SpecialTopics 2017;3(2):136-42.
•    Utku B., Akin S. Eccentric Exercises and Its Role in Preventing Sports Injuries. Turkiye Klinikleri J Sports Med-Special Topics 2017;3(3):233-39.
•    Akin Ş., Demirel H.A. Skeletal Muscle Satellite Cells and Their Role in Muscle Regeneration. Turkiye Klinikleri J Sports Med-Special Topics 2017;3(3):227-32.
•   Akin S., Naito H., Ogura Y., Ichinoseki-Sekine N., Kurosaka M., Kakigi R., Demirel H.A.Short-term treadmill exercise in a cold environment does not induce adrenal Hsp72 and Hsp25 expression. J Physiol Sci, 2017, 67(3):407-413.
•    Utku B., Dönmez G., Erişgen G., Akin Ş., Demirel H.A., Korkusuz F., Doral M.N. Meloxicam and diclofenac do not change VEGF and PDGF-ABserum levels of platelet-rich plasma. Turk J Med Sci. 2017, 47(2):570-576.
•   Donmez G., Doral M.N. Suljevic S., Sargon M.F. Bilgili H., Demirel H.A. Effects of immobilization and whole-body vibration on rat serum Type I collagen turnover. Acta Orthop Traumatol Turc. 2016, 50(4):452-7.
•   Gucuk Ipek, E., Akin-Suljevic, S., Kafes, H., Basyigit, F., Karalok, N., Guray, Y., Dinc Asarcikli, L., Acar, B., Demirel, H. Evaluation of galectin-3 levels in acute coronary syndrome. Annales de Cardiologie et d'angeiologie, 2015; 65(1):26-30.

Links

About

Research Infrastructure

•   -80°C Freezer (Thermo scientific, Forma 8800 Series)
•   -20°C Deep Freeze (Thermo scientific, Forma)
•   Snow Machine (Scotsman AF 80)
•   Dry Heating Block (VLM HP2)
•   Homogenizer (Bullet Blender Storm, Next Advance)
•   Refrigerated Centrifuge (Hermle Z 323 K)
•   Microplate reader  (SpectraMax i3x, Molecular Devices)
•   Gel Electrophoresis System (BioRad 16x20 and miniblot system)
•   Semi-Dry Transfer System (Trans-Blot Turbo, BioRad)
•   Chemiluminescence Imaging device (BIO-RAD ChemiDoc TMMP Imaging System and Flour Chem E Protein Simple)
•   Real Time PCR (ViiATM 7 Real-Time PCR system, Applied Biosystems, Foster City, CA)
•   Thermal Cycling Device (Veriti®, Applied Biosystems, Foster City, CA)
•   Cryotome (Shandon Cryotome SME)

Researchers

  • Professor Haydar Demirel, M.D. Ph.D.
  • Associate Professor Senay Akin
  • Assistant Profesor Inci Tugce Çolluoglu (Karabuk University, Faculty of Medicine, Department of Cardiology,)
  • Doctor Levend Karaçoban, M.D., Ph.D. (Department of Sports Medicine, Faculty of Medicine)
  • Ümit Hayta (Ph. D. student)
  • Research Assistant İrem Güngör (Ph. D. student)
  • Doctor Yigitcan Menderes, M.D. (Department of Sports Medicine, Faculty of Medicine)
  • Research Assistant Kagan Mert Gulec (Master student)
  • Eda Aytekin (trainee, 2022, Bilkent University, Molecular Biology and Genetics Department)

Ongoing Projects

•   The Role of Tübitak 1001-Whole Body Vibration in Reversing Immobilization-Induced Skeletal Muscle Atrophy

•   Tübitak 1002- The Role of Exercise-Induced Hsp72 in Preventing Glucocorticoid-Induced Skeletal Muscle Atrophy

Completed Projects

• 014 D01 407 001 -501 (Support) The effect of long-term swimming exercise on heart muscle antioxidant defense mechanism and cytokines in young and old rats
•    TED-2017-15076 (Event Participation) The Effect of Whole Body Vibration and Immobilization on Protein Synthesis and Destruction Pathways
•   THD-2017-13130 (Fast Boost) Effect of Acute Long Term Nature Running on Plasma Myokine Levels
•   TYL-2016-8835 (Master's Degree) The Effect of Whole Body Vibration and Immobilization on Myosin Heavy Chain Profile and HSP72 Levels in Rat Skeletal Muscle

Publications

•   Akin S., Kubat GB., Guray U., Akin Y., Demirel HA. Possible Value of Galectin-3 on follow-up of cardiac remodeling during Glucocorticoid treatment. Journal of Biochemical and Molecular Toxicology. It has been accepted for publication.
•   Akin S., Kubat GB., Demirel HA. Exercise, mitochondrial biogenesis and disuse-induced atrophy. Turkish Journal of Sports Medicine. It has been accepted for publication.
•   Akin Ş., Ozerklig B., Demirel H.A. Exercise-Induced Rhabdomyolysis. Various Health Problems in Athletes. 1st Edition. Ankara: Turkey Clinics; 2020. p.13- 8.
•    Yargic MP., Torgutalp S., Akin S., Babayeva N., Torgutalp M., Demirel AH. Acute long-distance trail running increases serum IL-6, IL-15, and Hsp72 levels. Appl Physiol NutrMetab. 2018; 10.1139/apnm-2018-0520.
•    Akin Ş., Ozerklig B., Turkel I., Demirel H.A. Molecular Mechanisms Regulating Skeletal Muscle Mass. Turkish Journal of Sports Medicine, 10.5152/tjsm.2019.125.
•    Akin Ş., Donmez G., Ozdemir M., Demirel H.A. Does Whole Body Vibration Increase AMPK Activation Decreased by Immobilization? Turkish Journal of Sports Medicine 2018,10.5152/tjsm.2018.107.
•    Akin Ş., Demirel H.A. Sarcopenia and Exercise. Turkiye Klinikleri J Sports Med-SpecialTopics 2017;3(2):136-42.
•    Utku B., Akin S. Eccentric Exercises and Its Role in Preventing Sports Injuries. Turkiye Klinikleri J Sports Med-Special Topics 2017;3(3):233-39.
•    Akin Ş., Demirel H.A. Skeletal Muscle Satellite Cells and Their Role in Muscle Regeneration. Turkiye Klinikleri J Sports Med-Special Topics 2017;3(3):227-32.
•   Akin S., Naito H., Ogura Y., Ichinoseki-Sekine N., Kurosaka M., Kakigi R., Demirel H.A.Short-term treadmill exercise in a cold environment does not induce adrenal Hsp72 and Hsp25 expression. J Physiol Sci, 2017, 67(3):407-413.
•    Utku B., Dönmez G., Erişgen G., Akin Ş., Demirel H.A., Korkusuz F., Doral M.N. Meloxicam and diclofenac do not change VEGF and PDGF-ABserum levels of platelet-rich plasma. Turk J Med Sci. 2017, 47(2):570-576.
•   Donmez G., Doral M.N. Suljevic S., Sargon M.F. Bilgili H., Demirel H.A. Effects of immobilization and whole-body vibration on rat serum Type I collagen turnover. Acta Orthop Traumatol Turc. 2016, 50(4):452-7.
•   Gucuk Ipek, E., Akin-Suljevic, S., Kafes, H., Basyigit, F., Karalok, N., Guray, Y., Dinc Asarcikli, L., Acar, B., Demirel, H. Evaluation of galectin-3 levels in acute coronary syndrome. Annales de Cardiologie et d'angeiologie, 2015; 65(1):26-30.

Links