Pharmaceutical Biotechnology Department Research Laboratory

About

Research Infrastructure

• Leica Dmıl Brand Microscope (Inverted/Normal Polarizer)
• Computer System (Software, Lg Flatron F900b Monitor, Hp Deskjet 5550 Printer, Hard Disk Enclosure)
• Microscope Accessories
• Leica Microscope Lenses (4 Pieces): 10x/0.22, 20x/0.30, 40x/0.5, 100x/1.25
• Leica Microscope Eyepieces (10x/18, 2 pcs) Depth 0.02 Mm
• Thoma Brand Lam, Graticules Brand 100 X 0.1= 10 Mm
• Microscope Ruler, Microscope Cup Holder and Movement Mechanisma (520804 Leitz Wetslar Germany)
• Microscope Cell Culture Flask Holder (Temporarily given to Vaccine Institute Laboratories for a Tuseb Project Related to Covid-19 Vaccine and will be returned when the project is over)
• Laptop/Notebook with Spectrophotometer (Nanodrop 2200c, Thermo Scientific) and Software (Asus)
• Vertical Electrophoresis Unit (Protean® II Xi Cell, Mini-Protean® Tetra Cell, Biorad), Western Blot Unit (Mini-Protean® Trans-Blot® Module, Biorad) and Electrical Power Supply (Powerpac Basic, Biorad)
• Phase Contrast Inverst Fluorescent Microscope Leica Dmi 3000b And Imaging System (Software, Computer, Monitor And Printer)(Computer Defective, Imaging Software Program Needs Updating))
• Spectrophotometer Nanodrop 1000 And Software (Thermo Fisher Scientific) (Connected to Hp Computer (Hpw1907 Monitor, Hp Pavillion Hardware)
• Malvern Zetasizer Nanosizer-S and Software (Connected to the Same HP Computer as Nanodrop Device (Hpw1907 Monitor, HP Pavillion Hardware)
• Shaker Incubator (Bacterial Culture) (Certomat-Bst) ( B. Braun Biotech International)
• Extruder- (Lipex Biomembranes), Circulator Water Bath (Polystat Cole Palmer) and Nitrogen Regulator
• Microplate Spectrofluorimeter (Gemini Xs-Spectramax) And Computer (Scuba Monitor And Hardware)
• Epitel Voltohmmeter (Evomx, World Precision Instruments) and Electrode Systems
• Vertical Electrophoresis And Blotting System (Thermo 142) (Electrophoresis Cell, Accessories And Electrophoresis Power Supply (Ec 250-90))
• UV/Vis Transilluminator (Alpha Innotech Corporation) (Defective)
• Incubation Cabinet (40c and 500c) (Biolab-Large Volume Custom Made)
• Carbon Dioxide Incubator (Shellab )(Defective/Not Repaired)
• Biorad Low Pressure Chromatography Device (Pump Defective)
• Microcentrifuge (24 Tubes of 1.5 - 2.0 Ml Capacity) (Hettich Eba12)
• Ultracentrifuge (Optima) (Beckman Coulter)
• Centrifuge Allegra X12r (Beckman Coulter)
• Autoclave (Hirayama Hmc, Hiclave Hv-25l)
• Centrifuge (Centra Thermo Ec Ccl2)
• Laminar Safety Cabinet (Faster Bhg 2004)
• Horizontal Electrophoresis System (Thermo)
• Iblot Gel Transfer System (Invitrogen)
• Electrophoresis Power Supply (Consort)
• Shaker (Labnet Orbit 1000)
• Low Pressure Chromatography Device (Bio-Rad) (Defective)
• Dry Block Heater (Techne Driblok)
• Heater (Corning)
• Dry Block Heater (Cole Palmer)
• Horizontal Electrophoresis Unit (Bio-Rad Midicell Ec350)
• Heated Incubator, +500c (Velp)
• Solid Phase Extraction Manifold (Supelco Visiprep Pl)
• Ph Meter (Sartorius Professional Meter Pp20) (2 Pieces)
• Magnetic Stirrer (Isotex Sh-4c)
• Dry Block Heater (Techne Driblok)
• Heater (Corning)
• Dry Block Heater (Cole Palmer)
• Precision Balance (Sartorius Bp1215 Max 120 G)
• Precision Balance (Schimadzu Ax200)
• Desktop Shaker (Lab-Line)
• Vortex (Velp Scientifica) (2 Pieces)
• Vortex (Lab-Line)
• Water Bath (Labortechnic)
• Refrigerator (Arçelik +40c and -200c Partitioned)
• Refrigerator Bosch Ksu49631ne
• Deep Freeze (-20 oC)

Researchers

• Prof. Dr. Türkan Eldem (Head of the Department)
• Pharm. Özgün Fırat Düzenli (Research Assistant)

Ongoing Projects

• Hübap, Tay-2020-19016, High Resolution Investigation of Deep Structures of Tissue and Live Animals by Multiphoton Microscopy, Research Infrastructure Project, Coordinator: Prof. Dr. Müge Yemişçi Özkan and 15 Researchers (Researcher: Prof. Dr. Türkan Eldem) Project Start Date: 14.12.2020 –

Completed Projects

• Tübitak 1000 Project, Regenerative and Restorative Medicine R&D Strategy Document, Project Coordinator Prof. Dr. İbrahim Vargel, (Researcher: Prof. Dr. Türkan Eldem) (2017-2019)
• Hübap Tbt-2017-15820, 2nd Lipid Research Workshop, Project Coordinator: Assoc. Dr. Yeşim Er Öztaş, Researcher: Prof. Dr. Turkan Eldem (2017)
• Hübap Rapid Support Project, Thd-2017-15688, Production and Characterization of Human Interferon Gamma Molecule in Cell-Free Protein Synthesis System Project Coordinator Prof. Dr. Turkan Eldem; Project Researchers: Prof. Dr. A. Filiz Öner, Ar. See. pharmacist. Özgün F. Regular; (2017- 2019)
• Hübap Graduate Thesis Project, Tdk-2017-16102, Investigation of Pharmaceutical Protein Production by Cellular Protein Expression Methods, Project Coordinator Prof. Dr. Turkan Eldem; Project Researchers: Prof. Dr. A. Filiz Öner, Ar. See. pharmacist. Özgün F. Regular; 2017- 2020 (Closed).

Publications

• Duzenli O.F., Oner A.F, “Blood–Brain Barrier: Genomics, Proteomics, Disease Targets And Drug Delivery”, Nanotechnology Methods For Neurological Diseases And Brain Tumors: Drug Delivery Across The Blood–Brain Barrier, Eds. Gursoy-Ozdemir, Y., Bozdag-Pehlivan, S., Sekerdag, E., Elsevier, 2017. (Book Chapter)
• Duzenli Of, Okay S. Promoter Engineering For The Recombinant Protein Production In Prokaryotic Systems. AIMs Bioengineering. 2020; 7(2), 62-81(Compilation Article)
• Regular, Of. Eldem, T., Oner, Af. Undergraduate and Postgraduate Education in Pharmaceutical Biotechnology, International İvek Bio Congress 26-28 November 2018, Istanbul, Turkey (Oral Presentation)
• Regular, Of. Oner, Af. (September, 2018). Effect Of Rnase A Addition Step On Purity Of Plasmid Dna Encoding Human Interferon Gamma Protein For Cell Free Protein Synthesis [Poster]. 19th International Pharmaceutical Technology Symposium Antalya, Turkey (Poster)
• Eldem T, Yapar E, Çağan B, Advanced Treatment Medicinal Products: Pharmaceutical Quality Assurance System, Good Manufacturing Practices and Basic Principles in Quality Control, Turkish Journal of Pharmacopoeia 2018, 3 (3): 36-41.
• Eldem T. Ethical Requirements for Medical Device Clinical Research, Turkiye Klinikleri J Med Ethics Law Hist-Special Topics 2018;4(1):69-74 (Review Article)
• Eldem T., Eldem B., Ocular Drug, Gene And Cellular Delivery System And Advanced Therapy Medicinal Products/Okler Pharmaceuticals, Gene And Cellular Delivery Systems And Advanced Therapy Medicinals,Turk J Ophthalmol 2018; 48: 132-141, DOI: 10.4274/Tjo.32458, (Compilation Article, Turkish And English) Eldem
• Eldem T., Pharmaceutical Biotechnology and Advanced Treatment Medicinal Products, Life Sciences and Cleanroom Technologies Newspaper, Issue 18, January- February, 2020, Page 10 (Compilation Article)
• Turkish Pharmacopoeia 2017 (Authors In Order; Uslu B., ......Eldem T., Ve Other., Ministry of Health Publication No: 1098, Turkish Medicines and Medical Devices Agency Publication No: 21, Isbn: 978-975 -590-675-1 ) (6 Volumes) (Book)
• Turkish Pharmacopoeia Supplement 7.1 2018 (Authors in Alphabetical Order by Name; Aydın A., ......Eldem T., Ve Other., Ministry of Health Publication No: 1105, Turkish Medicines and Medical Devices Agency Publication No: 22, Isbn:978-975-590-690-4)(Volumes 1 and 2) (Book)
• Turkish Pharmacopoeia Supplement 7.2 2019 (Authors, Alphabetical Order by Name; Sağırlı AO.....Eldem T.,.. Etc. TR Ministry of Health Publication No: 1143, Turkish Medicines and Medical Devices Agency Publication No: 26, Isbn :978-975-590-733-8) (Book) (1 Volume)

Links

About

Research Infrastructure

• Leica Dmıl Brand Microscope (Inverted/Normal Polarizer)
• Computer System (Software, Lg Flatron F900b Monitor, Hp Deskjet 5550 Printer, Hard Disk Enclosure)
• Microscope Accessories
• Leica Microscope Lenses (4 Pieces): 10x/0.22, 20x/0.30, 40x/0.5, 100x/1.25
• Leica Microscope Eyepieces (10x/18, 2 pcs) Depth 0.02 Mm
• Thoma Brand Lam, Graticules Brand 100 X 0.1= 10 Mm
• Microscope Ruler, Microscope Cup Holder and Movement Mechanisma (520804 Leitz Wetslar Germany)
• Microscope Cell Culture Flask Holder (Temporarily given to Vaccine Institute Laboratories for a Tuseb Project Related to Covid-19 Vaccine and will be returned when the project is over)
• Laptop/Notebook with Spectrophotometer (Nanodrop 2200c, Thermo Scientific) and Software (Asus)
• Vertical Electrophoresis Unit (Protean® II Xi Cell, Mini-Protean® Tetra Cell, Biorad), Western Blot Unit (Mini-Protean® Trans-Blot® Module, Biorad) and Electrical Power Supply (Powerpac Basic, Biorad)
• Phase Contrast Inverst Fluorescent Microscope Leica Dmi 3000b And Imaging System (Software, Computer, Monitor And Printer)(Computer Defective, Imaging Software Program Needs Updating))
• Spectrophotometer Nanodrop 1000 And Software (Thermo Fisher Scientific) (Connected to Hp Computer (Hpw1907 Monitor, Hp Pavillion Hardware)
• Malvern Zetasizer Nanosizer-S and Software (Connected to the Same HP Computer as Nanodrop Device (Hpw1907 Monitor, HP Pavillion Hardware)
• Shaker Incubator (Bacterial Culture) (Certomat-Bst) ( B. Braun Biotech International)
• Extruder- (Lipex Biomembranes), Circulator Water Bath (Polystat Cole Palmer) and Nitrogen Regulator
• Microplate Spectrofluorimeter (Gemini Xs-Spectramax) And Computer (Scuba Monitor And Hardware)
• Epitel Voltohmmeter (Evomx, World Precision Instruments) and Electrode Systems
• Vertical Electrophoresis And Blotting System (Thermo 142) (Electrophoresis Cell, Accessories And Electrophoresis Power Supply (Ec 250-90))
• UV/Vis Transilluminator (Alpha Innotech Corporation) (Defective)
• Incubation Cabinet (40c and 500c) (Biolab-Large Volume Custom Made)
• Carbon Dioxide Incubator (Shellab )(Defective/Not Repaired)
• Biorad Low Pressure Chromatography Device (Pump Defective)
• Microcentrifuge (24 Tubes of 1.5 - 2.0 Ml Capacity) (Hettich Eba12)
• Ultracentrifuge (Optima) (Beckman Coulter)
• Centrifuge Allegra X12r (Beckman Coulter)
• Autoclave (Hirayama Hmc, Hiclave Hv-25l)
• Centrifuge (Centra Thermo Ec Ccl2)
• Laminar Safety Cabinet (Faster Bhg 2004)
• Horizontal Electrophoresis System (Thermo)
• Iblot Gel Transfer System (Invitrogen)
• Electrophoresis Power Supply (Consort)
• Shaker (Labnet Orbit 1000)
• Low Pressure Chromatography Device (Bio-Rad) (Defective)
• Dry Block Heater (Techne Driblok)
• Heater (Corning)
• Dry Block Heater (Cole Palmer)
• Horizontal Electrophoresis Unit (Bio-Rad Midicell Ec350)
• Heated Incubator, +500c (Velp)
• Solid Phase Extraction Manifold (Supelco Visiprep Pl)
• Ph Meter (Sartorius Professional Meter Pp20) (2 Pieces)
• Magnetic Stirrer (Isotex Sh-4c)
• Dry Block Heater (Techne Driblok)
• Heater (Corning)
• Dry Block Heater (Cole Palmer)
• Precision Balance (Sartorius Bp1215 Max 120 G)
• Precision Balance (Schimadzu Ax200)
• Desktop Shaker (Lab-Line)
• Vortex (Velp Scientifica) (2 Pieces)
• Vortex (Lab-Line)
• Water Bath (Labortechnic)
• Refrigerator (Arçelik +40c and -200c Partitioned)
• Refrigerator Bosch Ksu49631ne
• Deep Freeze (-20 oC)

Researchers

• Prof. Dr. Türkan Eldem (Head of the Department)
• Pharm. Özgün Fırat Düzenli (Research Assistant)

Ongoing Projects

• Hübap, Tay-2020-19016, High Resolution Investigation of Deep Structures of Tissue and Live Animals by Multiphoton Microscopy, Research Infrastructure Project, Coordinator: Prof. Dr. Müge Yemişçi Özkan and 15 Researchers (Researcher: Prof. Dr. Türkan Eldem) Project Start Date: 14.12.2020 –

Completed Projects

• Tübitak 1000 Project, Regenerative and Restorative Medicine R&D Strategy Document, Project Coordinator Prof. Dr. İbrahim Vargel, (Researcher: Prof. Dr. Türkan Eldem) (2017-2019)
• Hübap Tbt-2017-15820, 2nd Lipid Research Workshop, Project Coordinator: Assoc. Dr. Yeşim Er Öztaş, Researcher: Prof. Dr. Turkan Eldem (2017)
• Hübap Rapid Support Project, Thd-2017-15688, Production and Characterization of Human Interferon Gamma Molecule in Cell-Free Protein Synthesis System Project Coordinator Prof. Dr. Turkan Eldem; Project Researchers: Prof. Dr. A. Filiz Öner, Ar. See. pharmacist. Özgün F. Regular; (2017- 2019)
• Hübap Graduate Thesis Project, Tdk-2017-16102, Investigation of Pharmaceutical Protein Production by Cellular Protein Expression Methods, Project Coordinator Prof. Dr. Turkan Eldem; Project Researchers: Prof. Dr. A. Filiz Öner, Ar. See. pharmacist. Özgün F. Regular; 2017- 2020 (Closed).

Publications

• Duzenli O.F., Oner A.F, “Blood–Brain Barrier: Genomics, Proteomics, Disease Targets And Drug Delivery”, Nanotechnology Methods For Neurological Diseases And Brain Tumors: Drug Delivery Across The Blood–Brain Barrier, Eds. Gursoy-Ozdemir, Y., Bozdag-Pehlivan, S., Sekerdag, E., Elsevier, 2017. (Book Chapter)
• Duzenli Of, Okay S. Promoter Engineering For The Recombinant Protein Production In Prokaryotic Systems. AIMs Bioengineering. 2020; 7(2), 62-81(Compilation Article)
• Regular, Of. Eldem, T., Oner, Af. Undergraduate and Postgraduate Education in Pharmaceutical Biotechnology, International İvek Bio Congress 26-28 November 2018, Istanbul, Turkey (Oral Presentation)
• Regular, Of. Oner, Af. (September, 2018). Effect Of Rnase A Addition Step On Purity Of Plasmid Dna Encoding Human Interferon Gamma Protein For Cell Free Protein Synthesis [Poster]. 19th International Pharmaceutical Technology Symposium Antalya, Turkey (Poster)
• Eldem T, Yapar E, Çağan B, Advanced Treatment Medicinal Products: Pharmaceutical Quality Assurance System, Good Manufacturing Practices and Basic Principles in Quality Control, Turkish Journal of Pharmacopoeia 2018, 3 (3): 36-41.
• Eldem T. Ethical Requirements for Medical Device Clinical Research, Turkiye Klinikleri J Med Ethics Law Hist-Special Topics 2018;4(1):69-74 (Review Article)
• Eldem T., Eldem B., Ocular Drug, Gene And Cellular Delivery System And Advanced Therapy Medicinal Products/Okler Pharmaceuticals, Gene And Cellular Delivery Systems And Advanced Therapy Medicinals,Turk J Ophthalmol 2018; 48: 132-141, DOI: 10.4274/Tjo.32458, (Compilation Article, Turkish And English) Eldem
• Eldem T., Pharmaceutical Biotechnology and Advanced Treatment Medicinal Products, Life Sciences and Cleanroom Technologies Newspaper, Issue 18, January- February, 2020, Page 10 (Compilation Article)
• Turkish Pharmacopoeia 2017 (Authors In Order; Uslu B., ......Eldem T., Ve Other., Ministry of Health Publication No: 1098, Turkish Medicines and Medical Devices Agency Publication No: 21, Isbn: 978-975 -590-675-1 ) (6 Volumes) (Book)
• Turkish Pharmacopoeia Supplement 7.1 2018 (Authors in Alphabetical Order by Name; Aydın A., ......Eldem T., Ve Other., Ministry of Health Publication No: 1105, Turkish Medicines and Medical Devices Agency Publication No: 22, Isbn:978-975-590-690-4)(Volumes 1 and 2) (Book)
• Turkish Pharmacopoeia Supplement 7.2 2019 (Authors, Alphabetical Order by Name; Sağırlı AO.....Eldem T.,.. Etc. TR Ministry of Health Publication No: 1143, Turkish Medicines and Medical Devices Agency Publication No: 26, Isbn :978-975-590-733-8) (Book) (1 Volume)

Links